Antibodies, Proteins, Kits and Reagents for Life Science

324 988 ₸ Product size

100 µl 324 988 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Chromogranin A antibody [EP1030Y] (ab68271)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1030Y] to Chromogranin A
Suitable for: WB, IHC-P, ICC, Flow Cyt
Reacts with: Human

Product name

Anti-Chromogranin A antibody [EP1030Y]
See all Chromogranin A primary antibodies
Description

Rabbit monoclonal [EP1030Y] to Chromogranin A
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Chromogranin A (N terminal). The exact sequence is proprietary.
Positive control
- WB: SH-SY5Y cell lysate. IHC-P: Human pancreas tissue. ICC: SH-SY5Y
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EP1030Y
Isotype

IgG
Research areas

Western blot - Anti-Chromogranin A antibody [EP1030Y] (ab68271)

Anti-Chromogranin A antibody [EP1030Y] (ab68271) + SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate at 10 µg

Secondary
HRP conjugated Goat anti-rabbit at 1/2000 dilution

Predicted band size: 51 kDa
Observed band size: 90 kDa
why is the actual band size different from the predicted?
Immunocytochemistry - Anti-Chromogranin A antibody [EP1030Y] (ab68271)

Immunocytochemistry analysis of SH-SY5Y (human neuroblastoma epithelial cell) labeling Chromogranin A with purified ab68271 at 1/50 dilution (3 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.17 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EP1030Y] (ab68271)

Formalin/PFA-fixed paraffin-embedded human pancreas tissue stained for Chromogranin A with ab68271 at a 1/100 dilution in immunohistochemical analysis.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-Chromogranin A antibody [EP1030Y] (ab68271)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com