Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Nova1 antibody [EPR13847] (ab183024)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13847] to Nova1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Nova1 antibody [EPR13847]
    See all Nova1 primary antibodies

  • Description

    Rabbit monoclonal [EPR13847] to Nova1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Rat
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Mouse brain tissue, PC12 cells, Human cerebellum tissue lysates; Human gliocytoma tissue; Rat brain tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13847

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nova1 antibody [EPR13847] (ab183024)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nova1 antibody [EPR13847] (ab183024)

    Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling Nova1 using ab183024 at 1/500 dilution.  A HRP conjugated Rabbit IgG was used as secondary (prediluted). Counterstain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nova1 antibody [EPR13847] (ab183024)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nova1 antibody [EPR13847] (ab183024)

    Immunohistochemical analysis of paraffin-embedded human gliocytoma tissue labeling Nova1 using ab183024 at 1/500 dilution. HRP-conjugated Rabbit IgG was used as secondary (prediluted). Counterstain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-Nova1 antibody [EPR13847] (ab183024)
    Western blot - Anti-Nova1 antibody [EPR13847] (ab183024)
    All lanes : Anti-Nova1 antibody [EPR13847] (ab183024) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : PC12 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 52 kDa

  • Western blot - Anti-Nova1 antibody [EPR13847] (ab183024)
    Western blot - Anti-Nova1 antibody [EPR13847] (ab183024)
    Anti-Nova1 antibody [EPR13847] (ab183024) at 1/5000 dilution + Human cerebellum lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution (Goat Anti-Rabbit IgG HRP)

    Predicted band size: 52 kDa

  • Anti-Nova1 antibody [EPR13847] (ab183024)
    Anti-Nova1 antibody [EPR13847] (ab183024)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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