Anti-Nova1 antibody (ab94511)
Key features and details
- Rabbit polyclonal to Nova1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Nova1 antibody
See all Nova1 primary antibodies -
Description
Rabbit polyclonal to Nova1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Nova1 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab105633) -
Positive control
- Recombinant Human Nova1 protein (ab114695) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; MCF7; A431.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatDogZebrafishApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).Target
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Function
May regulate RNA splicing or metabolism in a specific subset of developing neurons. -
Tissue specificity
Brain. -
Sequence similarities
Contains 3 KH domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 490632 Dog
- Entrez Gene: 4857 Human
- Entrez Gene: 664883 Mouse
- Entrez Gene: 298992 Rat
- Omim: 602157 Human
- SwissProt: P51513 Human
- SwissProt: Q9JKN6 Mouse
- SwissProt: Q80WA4 Rat
see all -
Alternative names
- Neuro oncological ventral antigen 1 antibody
- Neuro-oncological ventral antigen 1 antibody
- NOVA 1 antibody
see all
Images
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Western blot - Anti-Nova1 antibody (ab94511)All lanes : Anti-Nova1 antibody (ab94511) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 27 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Datasheets and documents
References (0)
ab94511 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Nova1 antibody (ab94511)All lanes : Anti-Nova1 antibody (ab94511) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 27 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
