Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22537-249] to Chromogranin A - BSA and Azide free
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free
    See all Chromogranin A primary antibodies

  • Description

    Rabbit monoclonal [EPR22537-249] to Chromogranin A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Nauro-2a andPC-12whole cell lysates; human adrenal gland lysate. IHC-P: Human pancreas and adrenal gland tissue; mouse pancreas and adrenal gland tissue; rat pancreas and adrenal gland tissue. IHC-Fr: Mouse colon tissue; rat colon tissue.

  • General notes

    Ab256169 is the carrier-free version of ab254557. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256169 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Strong staining on rat adrenal medulla (PMID: 15615849) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254557 for 10 mins at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254557).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse adrenal medulla (PMID: 15615849) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254557 for 10 mins at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254557).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)

    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human adrenal medulla (PMID: 15615849) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254557 for 10 mins at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254557).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat pancreas islet (PMID: 22412953) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254557 for 10 mins at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254557).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse pancreas islet (PMID: 22412953) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254557 for 10 mins at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254557).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human pancreas islet (PMID: 22412953) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab254557 for 10 mins at RT.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254557).

  • Immunohistochemistry (Frozen sections) - Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)
    Immunohistochemistry (Frozen sections) - Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)

    Immunohistochemical analysis of frozen section of rat colon tissue labeling Chromogranin A with ab254557 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining on enteroendocrine cells of colon (PMID: 27287799) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254557).

  • Immunohistochemistry (Frozen sections) - Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)
    Immunohistochemistry (Frozen sections) - Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)

    Immunohistochemical analysis of frozen section of mouse colon tissue labeling Chromogranin A with ab254557 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining on enteroendocrine cells of colon (PMID: 27287799) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254557).

  • Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)
    Anti-Chromogranin A antibody [EPR22537-249] - BSA and Azide free (ab256169)

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