Anti-Chromogranin A antibody [EP1031Y] (ab52983)
![Anti-Chromogranin A antibody [EP1031Y] (ab52983)](https://www.abcam.com/ps/products/52/ab52983/Images/ab52983-65557-anti-chromogranin-a-antibody-ep1031y-immunohistochemistry.jpg "Anti-Chromogranin A antibody [EP1031Y] (ab52983)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1031Y] to Chromogranin A
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Overview
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Product name
Anti-Chromogranin A antibody [EP1031Y]
See all Chromogranin A primary antibodies -
Description
Rabbit monoclonal [EP1031Y] to Chromogranin A -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Rat
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Immunogen
Synthetic peptide within Human Chromogranin A (C terminal). The exact sequence is proprietary.
(Peptide available asab138920) -
Positive control
- PC12 cytoplasmic cell lysate Human pancreatic tissue
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Dissociation constant (KD)
KD = 6.90 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1031Y -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EP1031Y] (ab52983)
ab52983, at a 1/250 dilution, staining Human Chromogranin A in Pancreas using Immunohistochemistry, Paraffin Emebedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-Chromogranin A antibody [EP1031Y] (ab52983)](https://www.abcam.com/ps/products/52/ab52983/Images/ab52983-65558-anti-chromogranin-a-antibody-ep1031y-western-blot.jpg)
Western blot - Anti-Chromogranin A antibody [EP1031Y] (ab52983)Anti-Chromogranin A antibody [EP1031Y] (ab52983) at 1/1000000 dilution + PC12 cell lysate at 10 µg
Secondary
goat anti-rabbit HRP labelled at 1/2000 dilution
Predicted band size: 51 kDa
Observed band size: 80-90 kDa why is the actual band size different from the predicted?
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![OI-RD Scanning - Anti-Chromogranin A antibody [EP1031Y] (ab52983)](https://www.abcam.com/ps/products/52/ab52983/Images/ab52983-65556-anti-chromogranin-a-antibody-ep1031y.jpg)
OI-RD Scanning - Anti-Chromogranin A antibody [EP1031Y] (ab52983) -
![Anti-Chromogranin A antibody [EP1031Y] (ab52983)](https://www.abcam.com/ps/products/52/ab52983/Images/ab52983-4-benefits-of-recombinant-antibodies.png)
Anti-Chromogranin A antibody [EP1031Y] (ab52983)
