Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Chromogranin A antibody [EPR22537-249] (ab254557)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22537-249] to Chromogranin A
Suitable for: WB, IHC-P, IHC-Fr
Reacts with: Mouse, Rat, Human

Product name

Anti-Chromogranin A antibody [EPR22537-249]
See all Chromogranin A primary antibodies
Description

Rabbit monoclonal [EPR22537-249] to Chromogranin A
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Neuro-2a and PC-12 whole cell lysates; human adrenal gland lysate. IHC-P: Human, mouse and rat pancreas and adrenal gland tissues. IHC-Fr: Mouse and rat colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22537-249
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] (ab254557)

Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by a rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse adrenal medulla (PMID: 15615849) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254557 for 10 mins at RT.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Western blot - Anti-Chromogranin A antibody [EPR22537-249] (ab254557)

Anti-Chromogranin A antibody [EPR22537-249] (ab254557) at 1/1000 dilution + Human adrenal gland tissue lysate at 20 µg

Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 52 kDa
Observed band size: 75 kDa
why is the actual band size different from the predicted?

Exposure time: 48 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 14734658, 21052719, 24260544).
Western blot - Anti-Chromogranin A antibody [EPR22537-249] (ab254557)

All lanes : Anti-Chromogranin A antibody [EPR22537-249] (ab254557) at 1/1000 dilution

Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line0 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 52 kDa
Observed band size: 100,20,75,85 kDa why is the actual band size different from the predicted?

Exposure time: 8 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 14734658, 21052719,24260544).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] (ab254557)

Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Strong staining on rat adrenal medulla (PMID: 15615849) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254557 for 10 mins at RT.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] (ab254557)

Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human adrenal medulla (PMID: 15615849) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254557 for 10 mins at RT.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] (ab254557)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat pancreas islet (PMID: 22412953) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254557 for 10 mins at RT.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] (ab254557)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse pancreas islet (PMID: 22412953) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254557 for 10 mins at RT.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin A antibody [EPR22537-249] (ab254557)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Chromogranin A with ab254557 at 1/5000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human pancreas islet (PMID: 22412953) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab254557 for 10 mins at RT.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemistry (Frozen sections) - Anti-Chromogranin A antibody [EPR22537-249] (ab254557)

Immunohistochemical analysis of frozen section of rat colon tissue labeling Chromogranin A with ab254557 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining on enteroendocrine cells of colon (PMID: 27287799) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-Chromogranin A antibody [EPR22537-249] (ab254557)

Immunohistochemical analysis of frozen section of mouse colon tissue labeling Chromogranin A with ab254557 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining on enteroendocrine cells of colon (PMID: 27287799) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Anti-Chromogranin A antibody [EPR22537-249] (ab254557)

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