Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)
![Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)](https://www.abcam.com/ps/products/177/ab177466/Images/ab177466-384464-anti-coilin-antibody-epr7843n-n-terminal-western-blot-hela-jurkat.jpg "Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7843(N)] to Coilin - N-terminal
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Coilin antibody [EPR7843(N)] - N-terminal
See all Coilin primary antibodies -
Description
Rabbit monoclonal [EPR7843(N)] to Coilin - N-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide within Human Coilin aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P38432 -
Positive control
- WB: Jurkat, HeLa, MCF7 and HepG2 cell lysates. Flow Cyt: Jurkat cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7843(N) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)All lanes : Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : COIL knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab177466 observed at 75 kDa. Red - loading control, ab181602 observed at 37 kDa.
ab177466 Anti-Coilin antibody [EPR7843(N)] - N-terminal was shown to specifically react with Coilin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261757 (knockout cell lysate ab257251) was used. Wild-type and Coilin knockout samples were subjected to SDS-PAGE. ab177466 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry - Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)](https://www.abcam.com/ps/products/177/ab177466/Images/ab177466-205802-anti-coilin-antibody-epr7843n-n-terminal-flow-cytometry.jpg)
Flow Cytometry - Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)Flow cytometric analysis of permeabilized Jurkat cells labeling Coilin (red) with ab177466 at 1/100 dilution or a rabbit IgG (negative) (green).
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![Western blot - Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)](https://www.abcam.com/ps/products/177/ab177466/Images/ab177466-205801-anti-coilin-antibody-epr7843n-n-terminal-western-blot.jpg)
Western blot - Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)All lanes : Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 63 kDa
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![Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)](https://www.abcam.com/ps/products/177/ab177466/Images/ab177466-4-benefits-of-recombinant-antibodies.png)
Anti-Coilin antibody [EPR7843(N)] - N-terminal (ab177466)
