Anti-Neuroglycan C antibody (ab31946)
Key features and details
- Rabbit polyclonal to Neuroglycan C
- Suitable for: ICC/IF, IHC-FoFr, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Neuroglycan C antibody
See all Neuroglycan C primary antibodies -
Description
Rabbit polyclonal to Neuroglycan C -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-FoFr, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Mouse Neuroglycan C.
Read Abcam's proprietary immunogen policy (Peptide available as ab32755.) -
Positive control
- This antibody gave a positive signal in the following lysates: Spinal Cord (Mouse) Tissue Lysate Spinal Cord (Rat) Lysate. Brain (Mouse) Tissue Lysate Brain (Rat) Tissue Lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab31946 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF IHC-FoFr WB Application notesICC/IF: Use at a concentration of 1 µg/ml.
IHC-FoFr: 1/300 (See abreview for more details)
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 60,45 kDa (predicted molecular weight: 60,58,48 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
May function as a growth and differentiation factor involved in neuritogenesis. May induce ERBB3 activation. -
Tissue specificity
Restricted to brain (at protein level). -
Sequence similarities
Contains 1 EGF-like domain. -
Developmental stage
Expressed in brain of 3 months, 5 and 10-year-old individuals. -
Post-translational
modificationsN-glycosylated.
O-glycosylated; contains chondroitin sulfate glycans. Part-time proteoglycan, expressed in part as a proteoglycan exhibiting chondroitin sulfate glycans and in part as a non-proteoglycan form. The relative amount of both forms depends on tissues and tissues maturation.
Phosphorylated; in intracellular and extracellular parts. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus membrane. In neurons, localizes to synaptic junctions (By similarity). Also detected in the endoplasmic reticulum and the Golgi (By similarity). Partially enriched in lipid rafts. - Information by UniProt
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Database links
- Entrez Gene: 10675 Human
- Entrez Gene: 29873 Mouse
- Entrez Gene: 50568 Rat
- Omim: 606775 Human
- SwissProt: O95196 Human
- SwissProt: Q71M36 Mouse
- SwissProt: Q9ERQ6 Rat
- Unigene: 45127 Human
see all -
Alternative names
- Acidic leucine rich EGF like domain containing brain protein antibody
- Acidic leucine-rich EGF-like domain-containing brain protein antibody
- Caleb antibody
see all
Images
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Neuroglycan C antibody (ab31946)
ab31946 staining Neuroglycan C in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used at 1/1000 dilution, as secondary.
This image is courtesy of an Abreview submitted by Dr Sophie Pezet.
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Western blot - Anti-Neuroglycan C antibody (ab31946)All lanes : Anti-Neuroglycan C antibody (ab31946) at 1 µg/ml
Lane 1 : Mouse Spinal Cord Lysate
Lane 2 : Rat Spinal Cord Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 60,58,48 kDa
Observed band size: 45,60 kDa why is the actual band size different from the predicted?
It is impossible to conclude from the data whether the higher observed band is Neuroglycan C isoform 3 (60 kDa) or Neuroglycan C isoform 1 (57 kDa). The epitope for this antibody is present in all 3 isoforms of the Neuroglycan C protein. We suspect the lower band is Isoform 2. -
Western blot - Anti-Neuroglycan C antibody (ab31946)All lanes : Anti-Neuroglycan C antibody (ab31946) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 60,58,48 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Neuroglycan C antibody (ab31946)ICC/IF image of ab31946 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31946, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Protocols
Datasheets and documents
References (1)
ab31946 has been referenced in 1 publication.
- Finelli MJ et al. The epilepsy-associated protein TBC1D24 is required for normal development, survival and vesicle trafficking in mammalian neurons. Hum Mol Genet 28:584-597 (2019). PubMed: 30335140
Images
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Neuroglycan C antibody (ab31946)
ab31946 staining Neuroglycan C in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG was used at 1/1000 dilution, as secondary.
This image is courtesy of an Abreview submitted by Dr Sophie Pezet.
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Western blot - Anti-Neuroglycan C antibody (ab31946)All lanes : Anti-Neuroglycan C antibody (ab31946) at 1 µg/ml
Lane 1 : Mouse Spinal Cord Lysate
Lane 2 : Rat Spinal Cord Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 60,58,48 kDa
Observed band size: 45,60 kDa why is the actual band size different from the predicted?
It is impossible to conclude from the data whether the higher observed band is Neuroglycan C isoform 3 (60 kDa) or Neuroglycan C isoform 1 (57 kDa). The epitope for this antibody is present in all 3 isoforms of the Neuroglycan C protein. We suspect the lower band is Isoform 2. -
Western blot - Anti-Neuroglycan C antibody (ab31946)All lanes : Anti-Neuroglycan C antibody (ab31946) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 60,58,48 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
-
Immunocytochemistry/ Immunofluorescence - Anti-Neuroglycan C antibody (ab31946)ICC/IF image of ab31946 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31946, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
