Anti-Coilin antibody [Pdelta] (ab11822)
![Anti-Coilin antibody [Pdelta] (ab11822)](https://www.abcam.com/ps/products/11/ab11822/Images/ab11822-19107-anti-coilin-antibody-pdelta-immunofluorescence.jpg "Anti-Coilin antibody [Pdelta] (ab11822)")
Key features and details
- Mouse monoclonal [Pdelta] to Coilin
- Suitable for: ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-Coilin antibody [Pdelta]
See all Coilin primary antibodies -
Description
Mouse monoclonal [Pdelta] to Coilin -
Host species
Mouse -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Recombinant fragment corresponding to Human Coilin (C terminal).
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Epitope
Monoclonal Anti-coilin reacts with an epitope located between amino acids 363 and 481 of human coilin. -
Positive control
- HeLa nuclear extract.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Pdelta -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Coilin antibody [Pdelta] (ab11822)ICC/IF image of ab11822 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11822, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
