Rhodamine Phalloidin Reagent (ab235138)
Key features and details
- Assay type: Cell-based (qualitative)
- Detection method: Fluorescent
- Platform: Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Rhodamine Phalloidin Reagent
See all F-actin kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Product overview
Rhodamine Phalloidin Reagent ab235138 is one of a series of phalloidin conjugates that are used to stain actin filaments in fluoresence microscopy. The tetramethyl rhodamine dye can be easily detected with a fluorescent microscope at Ex/Em = 546/575 nm.
Phalloidin conjugates are convenient probes for labeling, identifying and quantitating animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.
Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488, Phalloidin-iFluor 647, Phalloidin-iFluor 594, and Phalloidin-iFluor 555, search the website to see all phallodin conjugates, or read the phalloidin staining protocol.
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Notes
Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.
When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 300 tests Rhodamine Phalloidin Conjugate 1 x 300 tests -
Research areas
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Alternative names
- actin filament
- f actin
- Filamentous actin
