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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin

Anti-beta Actin antibody (ab223353)

Price and availability

271 382 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-beta Actin antibody (ab223353)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to beta Actin
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-beta Actin antibody
    See all beta Actin primary antibodies
  • Description

    Rabbit polyclonal to beta Actin
  • Host species

    Rabbit
  • Specificity

    Predicted molecular weight at ~41.7kDa. A double band may be observed.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human beta Actin (internal sequence).
    Database link: P60709

  • Positive control

    • WB: HeLa and HEK-293T lysates. ICC/IF: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin

Images

  • Western blot - Anti-beta Actin antibody (ab223353)
    Western blot - Anti-beta Actin antibody (ab223353)
    All lanes : Anti-beta Actin antibody (ab223353) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HEK-294T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG HRP at 1/2000 dilution

    Predicted band size: 41.7 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?



    Block: 5% milk + TBST for 1 hour at RT.

    Primary incubation: 1 hour at room temperature

  • Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody (ab223353)
    Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody (ab223353)

    PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin (green) using ab223353 at a dilution of 1/100 in ICC/IF (centre panel). The nuclear conterstain was DAPI (blue)(left panel). The merged images are shown in the right-hand panel.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-beta Actin antibody (ab223353)

  •  
  • Product image

    Anti-beta Actin antibody [EPR21242] (ab241153)

    Applications: ICC/IF, WB

  •  
  • Product image

    Anti-beta Actin antibody [mAbcam 8226] - BSA and Azide free (ab264083)

    Applications: Flow Cyt, ICC, ICC/IF, IHC-Fr, IHC-FrFl, IHC-P, IP, WB

  •  
  • Product image

    Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226)

    Applications: ICC/IF, IHC-P, WB

  •  
  • Product image

    HRP Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab20272)

    Applications: WB

  •  
  • Product image

    Anti-beta Actin antibody (ab8227)

    Applications: ICC/IF, IHC-P, WB

  •  
  • Product image

    Anti-beta Actin antibody [mAbcam 8224] - Loading Control (ab8224)

    Applications: Flow Cyt, IHC-P, WB

  •  
  • Product image

    Anti-beta Actin antibody [EPR21241] (ab213262)

    Applications: ICC/IF, WB

  •  
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    Anti-beta Actin antibody [SP124] - BSA and Azide free (ab242387)

    Applications: Flow Cyt, ICC/IF, IHC-P, WB

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