Anti-Profilin 2 antibody - C-terminal (ab191054)
Key features and details
- Rabbit polyclonal to Profilin 2 - C-terminal
- Suitable for: Flow Cyt, WB, IHC-P, ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Profilin 2 antibody - C-terminal
See all Profilin 2 primary antibodies -
Description
Rabbit polyclonal to Profilin 2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IHC-P, ICCmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Cynomolgus monkey, Orangutan
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Immunogen
Synthetic peptide corresponding to Human Profilin 2 aa 100 to the C-terminus (C terminal).
Database link: P35080 -
Positive control
- WB: human placenta tissue, rat brain tissue and mouse brain tissue; IHC-P: Human intestinal cancer tissue; Flow Cyt: A431 cells; ICC: SH-SY5Y cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-Profilin 2 antibody - C-terminal (ab191054)
Immunocytochemistry analysis of SH-SY5Y cells labelling Profilin 2 with ab191054 at 5 µg/mL overnight at 4°C.. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent and the cells were blocked with 10% goat serum. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 2 antibody - C-terminal (ab191054)Immunohistochemical analysis of paraffin-embedded human tonsil tissue staining Profilin 2 with ab191054 at 1µg/ml, followed by a biotinylated goat anti-rabbit IgG secondary antibody. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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Western blot - Anti-Profilin 2 antibody - C-terminal (ab191054)All lanes : Anti-Profilin 2 antibody - C-terminal (ab191054) at 0.5 µg/ml
Lane 1 : Human placenta tissue lysates
Lane 2 : Rat brain tissue lysates
Lane 3 : Mouse brain tissue lysates
Secondary
All lanes : Goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 2 antibody - C-terminal (ab191054)Immunohistochemical analysis of paraffin-embedded human intestincal cancer tissue staining Profilin 2 with ab191054 at 1µg/ml, followed by a biotinylated goat anti-rabbit IgG secondary antibody. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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Flow Cytometry - Anti-Profilin 2 antibody - C-terminal (ab191054)Flow Cytometry analysis of A431 cells labeling Profilin 2 with ab191054 (1μg/1x106 cells), DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody (blue). Isotype control antibody was rabbit IgG (1μg/1x106) (green). An unlabelled samples was used as a second control (red). The cells were blocked with 10% normal goat serum
