Anti-gamma Actin antibody [2A3] (ab123034)
![Anti-gamma Actin antibody [2A3] (ab123034)](https://www.abcam.com/ps/products/123/ab123034/Images/ab123034-343910-AntigammaActinantibody-2A3-immunocytochemistry-dermal-fibroblasts-human.jpg "Anti-gamma Actin antibody [2A3] (ab123034)")
Key features and details
- Mouse monoclonal [2A3] to gamma Actin
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human, Pig
- Isotype: IgG2b
Overview
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Product name
Anti-gamma Actin antibody [2A3]
See all gamma Actin primary antibodies -
Description
Mouse monoclonal [2A3] to gamma Actin -
Host species
Mouse -
Specificity
This antibody does not cross react with other actin isoforms. The epitope recognized by ab123034 is highly conserved and the antibody is therefore expected to react with many other species.
This antibody is highly specific for gamma-cytoplasmic actin (γ-actin 1; encoded by ACTG1). It does not cross-react with the gamma-actin isoform expressed in the enteric smooth muscle tissues (γ-actin 2; encoded by ACTG2).
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Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanPig
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Immunogen
Synthetic peptide (Ac-
EEEIAALVI
-COOH) with an acetylated N-terminus and conjugated to KLH by a Cysteine residue linker, corresponding to N terminal amino acids 2-10 of Human gamma Actin -
Positive control
- ICC/IF: Human dermal fibroblast cells. IHC-P: Human small intestine and heart tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
2A3 -
Myeloma
NS0 -
Isotype
IgG2b -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-gamma Actin antibody [2A3] (ab123034)
Human dermal fibroblasts stained for gamma Actin (green) using ab123034 at 1/500 dilution in ICC/IF.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Actin antibody [2A3] (ab123034)](https://www.abcam.com/ps/products/123/ab123034/Images/ab123034-169205-anti-gamma-actin-antibody-2a3-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Actin antibody [2A3] (ab123034)Immunohistochemical analysis of paraffin embedded Human small intestine tissue labelled with Anti-gamma Actin antibody [2A3] at 5 μg/ml
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Actin antibody [2A3] (ab123034)](https://www.abcam.com/ps/products/123/ab123034/Images/ab123034-391165-anti-gamma-actin-antibody-2a3-immunohistochemistry-pig-smooth-muscle.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Actin antibody [2A3] (ab123034)ab123034 (1:1000 dilution) stainign gamma Actin in the epithelial villi and smooth muscle cells in swine colon.
