Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-gamma Actin antibody [2A3] (ab123034)

Key features and details

  • Mouse monoclonal [2A3] to gamma Actin
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human, Pig
  • Isotype: IgG2b

Overview

  • Product name

    Anti-gamma Actin antibody [2A3]
    See all gamma Actin primary antibodies

  • Description

    Mouse monoclonal [2A3] to gamma Actin

  • Host species

    Mouse

  • Specificity

    This antibody does not cross react with other actin isoforms. The epitope recognized by ab123034 is highly conserved and the antibody is therefore expected to react with many other species.

    This antibody is highly specific for gamma-cytoplasmic actin (γ-actin 1; encoded by ACTG1). It does not cross-react with the gamma-actin isoform expressed in the enteric smooth muscle tissues (γ-actin 2; encoded by ACTG2).

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    Pig
    See all applications and species data

  • Immunogen

    Synthetic peptide (Ac-

    EEEIAALVI

    -COOH) with an acetylated N-terminus and conjugated to KLH by a Cysteine residue linker, corresponding to N terminal amino acids 2-10 of Human gamma Actin
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • ICC/IF: Human dermal fibroblast cells. IHC-P: Human small intestine and heart tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    2A3

  • Myeloma

    NS0

  • Isotype

    IgG2b

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Actin antibody [2A3] (ab123034)
    Immunocytochemistry/ Immunofluorescence - Anti-gamma Actin antibody [2A3] (ab123034)

    Human dermal fibroblasts stained for gamma Actin (green) using ab123034 at 1/500 dilution in ICC/IF.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Actin antibody [2A3] (ab123034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Actin antibody [2A3] (ab123034)

    Immunohistochemical analysis of paraffin embedded Human small intestine tissue labelled with Anti-gamma Actin antibody [2A3] at 5 μg/ml

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Actin antibody [2A3] (ab123034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Actin antibody [2A3] (ab123034)

    ab123034 (1:1000 dilution) stainign gamma Actin in the epithelial villi and smooth muscle cells in swine colon.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-gamma Actin antibody [2A3] (ab123034)

© 2021 Astana Biomed Group LLP. All rights reserved.