Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 03, 2021

Anti-gamma Actin antibody [EPR19353-2] (ab200046)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19353-2] to gamma Actin
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-gamma Actin antibody [EPR19353-2]
See all gamma Actin primary antibodies
Description

Rabbit monoclonal [EPR19353-2] to gamma Actin
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human Gamma actin (ACTG1) recombinant protein; Jurkat, A549, HeLa, C6, PC-12 and NIH/3T3 whole cell lysates; Human fetal spleen, fetal kidney and fetal brain lysates; Mouse kidney and spleen lysates; Rat kidney, spleen and brain lysates. IP: HeLa whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19353-2
Isotype

IgG
Research areas

Western blot - Anti-gamma Actin antibody [EPR19353-2] (ab200046)

All lanes : Anti-gamma Actin antibody [EPR19353-2] (ab200046) at 1/1000 dilution

Lane 1 : Human Gamma actin (ACTG1) recombinant protein at 0.01 µg
Lane 2 : Human Beta actin (ACTB) recombinant protein at 0.02 µg
Lane 3 : Human Gamma-2 actin(ACTG2) recombinant protein at 0.02 µg
Lane 4 : Human Alpha-cardiac actin (ACTC) recombinant protein at 0.02 µg
Lane 5 : Human Alpha-actin-2 (ACTA2) recombinant protein at 0.01 µg
Lane 6 : Human Alpha-actin-1 (ACTA1) recombinant protein at 0.01 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 42 kDa
Observed band size: 22 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Human Gamma actin (ACTG1) contains aa2-182 with His-tag.

Human Beta actin (ACTB) contains aa1-100 with GST and His tag.

Human Gamma-2 actin(ACTG2) contains aa1-200 with His-tag.

Human Alpha-cardiac actin (ACTC) contains aa1-200 with His-tag.

Human Alpha-actin-2 (ACTA2) contains aa3-253 with His-tag.

Human Alpha-actin-1 (ACTA1) contains aa3-200 with His-tag
Western blot - Anti-gamma Actin antibody [EPR19353-2] (ab200046)

All lanes : Anti-gamma Actin antibody [EPR19353-2] (ab200046) at 1/1000 dilution

Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 2 : A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-gamma Actin antibody [EPR19353-2] (ab200046)

All lanes : Anti-gamma Actin antibody [EPR19353-2] (ab200046) at 1/1000 dilution

Lane 1 : Human fetal spleen lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal brain lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1/3: 3 minutes; Lane 2: 30 seconds.
Western blot - Anti-gamma Actin antibody [EPR19353-2] (ab200046)

All lanes : Anti-gamma Actin antibody [EPR19353-2] (ab200046) at 1/1000 dilution

Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 4 : Mouse kidney lysate
Lane 5 : Mouse spleen lysate
Lane 6 : Rat brain lysate
Lane 7 : Rat kidney lysate
Lane 8 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1-3: 30 seconds; Lane 4/5: 3 minutes; Lane 6-8: 1 minute
Immunoprecipitation - Anti-gamma Actin antibody [EPR19353-2] (ab200046)

gamma Actin was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab200046 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab200046 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HeLa whole cell lysate, 10 μg (Input).

Lane 2: ab200046 IP in HeLa whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200046 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-gamma Actin antibody [EPR19353-2] (ab200046)

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