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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin

Anti-gamma Actin antibody (ab194952)

Price and availability

335 040 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-gamma Actin antibody (ab194952)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to gamma Actin
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-gamma Actin antibody
    See all gamma Actin primary antibodies
  • Description

    Rabbit polyclonal to gamma Actin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within gamma Actin aa 150-250 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P63261

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • K562 and HepG2 cell line lysates; 293 cell lysate transiently transfected with gamma Actin; Human Spleen tissue; HepG2 cells; K562 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin

Images

  • Western blot - Anti-gamma Actin antibody (ab194952)
    Western blot - Anti-gamma Actin antibody (ab194952)
    All lanes : Anti-gamma Actin antibody (ab194952) at 1/1000 dilution

    Lane 1 : K562 cell line lysate
    Lane 2 : HepG2 cell line lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 42 kDa

  • Western blot - Anti-gamma Actin antibody (ab194952)
    Western blot - Anti-gamma Actin antibody (ab194952)
    All lanes : Anti-gamma Actin antibody (ab194952) at 1/1000 dilution

    Lane 1 : 293 cell lysate non-transfected
    Lane 2 : 293 cell lysate transiently transfected with gamma Actin

    Lysates/proteins at 2 µg per lane.

    Predicted band size: 42 kDa

  • Flow Cytometry - Anti-gamma Actin antibody (ab194952)
    Flow Cytometry - Anti-gamma Actin antibody (ab194952)

    Flow cytometry of K562 cells using ab194952 at 1/10 (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-gamma Actin antibody (ab194952)
    Immunocytochemistry/ Immunofluorescence - Anti-gamma Actin antibody (ab194952)

    Immunofluorescent analysis of  HepG2 cells labeling gamma Actin with ab194952 at 1/10 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Actin antibody (ab194952)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-gamma Actin antibody (ab194952)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling gamma Actin with ab194952 at 1/100.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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