Anti-gamma Actin antibody (ab194952)
Key features and details
- Rabbit polyclonal to gamma Actin
- Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-gamma Actin antibody
See all gamma Actin primary antibodies -
Description
Rabbit polyclonal to gamma Actin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within gamma Actin aa 150-250 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P63261 -
Positive control
- K562 and HepG2 cell line lysates; 293 cell lysate transiently transfected with gamma Actin; Human Spleen tissue; HepG2 cells; K562 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-gamma Actin antibody (ab194952) at 1/1000 dilution
Lane 1 : K562 cell line lysate
Lane 2 : HepG2 cell line lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 42 kDa
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All lanes : Anti-gamma Actin antibody (ab194952) at 1/1000 dilution
Lane 1 : 293 cell lysate non-transfected
Lane 2 : 293 cell lysate transiently transfected with gamma Actin
Lysates/proteins at 2 µg per lane.
Predicted band size: 42 kDa
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Flow cytometry of K562 cells using ab194952 at 1/10 (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Immunofluorescent analysis of HepG2 cells labeling gamma Actin with ab194952 at 1/10 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI staining.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human spleen tissue labeling gamma Actin with ab194952 at 1/100.