Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP969Y] to LIMK2 - C-terminal
  • Suitable for: IHC-P, ICC/IF, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-LIMK2 antibody [EP969Y] - C-terminal
    See all LIMK2 primary antibodies

  • Description

    Rabbit monoclonal [EP969Y] to LIMK2 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human LIMK2 aa 600-700 (C terminal). The exact sequence is proprietary.
    Database link: P53671

  • Positive control

    • WB: Wild-type HAP1 cell lysate. Jurkat cell lysate. Ramos membrane lysate. IHC-P: Human ovary carcinoma tissue. ICC/IF: Ramos cells.

  • General notes

    Previously labelled as LIM kinase 2. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
    Western blot - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)

    Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
    Lane 2: LIMK2 (LIMK2) knockout HAP1 whole cell lysate (40 µg)
    Lane 3: Jurkat whole cell lysate (40 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab45165 observed at 72 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab45165 was shown to specifically recognize LIMK2 (LIMK2) in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed with knockout cells were examined. Wild-type and LIMK2 (LIMK2) knockout samples were subjected to SDS-PAGE. Ab45165 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)

    ab45165 staining LIMK2 in human ovary carcinoma by immunohistochemistry using paraffin embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-LIMK2 antibody [EP969Y] - Conformation-Specific (ab45165)
    Immunocytochemistry/ Immunofluorescence - Anti-LIMK2 antibody [EP969Y] - Conformation-Specific (ab45165)

    Immunocytochemistry/ Immunofluorescence analysis of Ramos cells labeling LIMK2 with ab45165 at 1/250. Goat anti rabbit IgG(Alexa Fluor® 488); ab150077 at 1/1000 dilution was used as the secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% tritonX-100. DAPI (blue) was used as a nuclear counterstain.

     

  • Western blot - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
    Western blot - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
    Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165) at 1/2000 dilution + Ramos (Human Burkitt's lymphoma cell line) membrane lysate

    Predicted band size: 72 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165) Image from Rice DS et al., PLoS One. 2012;7(10):e47168. doi: 10.1371/journal.pone.0047168. Epub 2012 Oct 5.; Fig 6.; October 5, 2012, PLoS ONE 7(10): e47168.

    Immunohistochemical analyis of mouse upper eyelid tissue, staining LIMK2 with ab45165 at 1/200 dilution. Staining was detected using DAB.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)
    Anti-LIMK2 antibody [EP969Y] - C-terminal (ab45165)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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