Anti-Profilin 1 antibody (ab92803)
Key features and details
- Rabbit polyclonal to Profilin 1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Profilin 1 antibody
See all Profilin 1 primary antibodies -
Description
Rabbit polyclonal to Profilin 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human Profilin 1 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab104286) -
Positive control
- Recombinant Human Profilin 1 protein (ab87760) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: NIH3T3; HeLa; HEK293; HuES7; HepG2; Jurkat; HAP1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseHumanAll applications RatCowPigApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).Target
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Function
Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. -
Sequence similarities
Belongs to the profilin family. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 5216 Human
- Entrez Gene: 18643 Mouse
- Entrez Gene: 64303 Rat
- Omim: 176610 Human
- SwissProt: P07737 Human
- SwissProt: P62962 Mouse
- SwissProt: P62963 Rat
- Unigene: 494691 Human
see all -
Alternative names
- Actin binding protein antibody
- ALS18 antibody
- Epididymis tissue protein Li 184a antibody
see all
Images
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Western blot - Anti-Profilin 1 antibody (ab92803)All lanes : Anti-Profilin 1 antibody (ab92803) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PFN1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 15 kDa
Observed band size: 15 kDaLanes 1 - 4: Merged signal (red and green). Green - ab92803 observed at 15 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab92803 was shown to specifically react with in wild-type HAP1 cells as signal was lost in PFN1 knockout cells. Wild-type and PFN1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab92803 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Profilin 1 antibody (ab92803)All lanes : Anti-Profilin 1 antibody (ab92803) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 90 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab92803 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Profilin 1 antibody (ab92803)All lanes : Anti-Profilin 1 antibody (ab92803) at 1 µg/ml
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PFN1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 15 kDa
Observed band size: 15 kDaLanes 1 - 4: Merged signal (red and green). Green - ab92803 observed at 15 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab92803 was shown to specifically react with in wild-type HAP1 cells as signal was lost in PFN1 knockout cells. Wild-type and PFN1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab92803 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Anti-Profilin 1 antibody (ab92803)All lanes : Anti-Profilin 1 antibody (ab92803) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 90 seconds
