Anti-Profilin 1 antibody [CL3524] (ab242369)
![Anti-Profilin 1 antibody [CL3524] (ab242369)](https://www.abcam.com/ps/products/242/ab242369/Images/ab242369-331669-anti-profilin-1-antibody-cl3524-immunohistochemistry.jpg "Anti-Profilin 1 antibody [CL3524] (ab242369)")
Key features and details
- Mouse monoclonal [CL3524] to Profilin 1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Profilin 1 antibody [CL3524]
See all Profilin 1 primary antibodies -
Description
Mouse monoclonal [CL3524] to Profilin 1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Profilin 1 aa 50 to the C-terminus.
Database link: P07737 -
Positive control
- WB: Control siRNA transfected U-251 MG cell lysate. HeLa, HEK-293, A431 and HepG2 cell lysate. IHC-P: Human fallopian tube, tonsil, liver, kidney and testis tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
CL3524 -
Isotype
IgG2a -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [CL3524] (ab242369)
Formalin-fixed, paraffin-embedded human kidney tissue stained for Profilin 1 with ab242369 at a 1:1000 dilution in immunohistochemical analysis.
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![Western blot - Anti-Profilin 1 antibody [CL3524] (ab242369)](https://www.abcam.com/ps/products/242/ab242369/Images/ab242369-331664-anti-profilin-1-antibody-cl3524-western-blot.jpg)
Western blot - Anti-Profilin 1 antibody [CL3524] (ab242369)All lanes : Anti-Profilin 1 antibody [CL3524] (ab242369) at 1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 3 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [CL3524] (ab242369)](https://www.abcam.com/ps/products/242/ab242369/Images/ab242369-331668-anti-profilin-1-antibody-cl3524-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [CL3524] (ab242369)Formalin-fixed, paraffin-embedded human liver tissue stained for Profilin 1 with ab242369 at a 1:1000 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [CL3524] (ab242369)](https://www.abcam.com/ps/products/242/ab242369/Images/ab242369-331667-anti-profilin-1-antibody-cl3524-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [CL3524] (ab242369)Formalin-fixed, paraffin-embedded human tonsil tissue stained for Profilin 1 with ab242369 at a 1:1000 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [CL3524] (ab242369)](https://www.abcam.com/ps/products/242/ab242369/Images/ab242369-331666-anti-profilin-1-antibody-cl3524-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [CL3524] (ab242369)Formalin-fixed, paraffin-embedded human fallopian tube tissue stained for Profilin 1 with ab242369 at a 1:1000 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [CL3524] (ab242369)](https://www.abcam.com/ps/products/242/ab242369/Images/ab242369-331670-anti-profilin-1-antibody-cl3524-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Profilin 1 antibody [CL3524] (ab242369)Formalin-fixed, paraffin-embedded human testis tissue stained for Profilin 1 with ab242369 at a 1:1000 dilution in immunohistochemical analysis.
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![Western blot - Anti-Profilin 1 antibody [CL3524] (ab242369)](https://www.abcam.com/ps/products/242/ab242369/Images/ab242369-331665-anti-profilin-1-antibody-cl3524-western-blot.jpg)
Western blot - Anti-Profilin 1 antibody [CL3524] (ab242369)All lanes : Anti-Profilin 1 antibody [CL3524] (ab242369) at 1 µg/ml
Lane 1 : Control siRNA transfected U-251 MG (Human brain glioma cell line) cell lysate
Lanes 2-3 : siRNA#1 transfected U-251 MG cell lysateLoading control is anti-GAPDH.
