Antibodies, Proteins, Kits and Reagents for Life Science

348 441 ₸ Product size

100 µl 348 441 ₸

Order now and get it on Thursday February 25, 2021

Alexa Fluor® 647 Anti-Profilin 1 antibody [EPR6304] (ab203146)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Alexa Fluor® 647 Rabbit monoclonal [EPR6304] to Profilin 1
Suitable for: ICC/IF, Flow Cyt, WB
Reacts with: Human
Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm

Product name

Alexa Fluor® 647 Anti-Profilin 1 antibody [EPR6304]
See all Profilin 1 primary antibodies
Description

Alexa Fluor® 647 Rabbit monoclonal [EPR6304] to Profilin 1
Host species

Rabbit
Conjugation

Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Tested applications

Suitable for: ICC/IF, Flow Cyt, WBmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide corresponding to Human Profilin 1 (C terminal).
Database link: P07737
Positive control
- IF/ICC: HeLa cells. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Alexa Fluor^® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor^® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor^® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor^® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Monoclonal
Clone number

EPR6304
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Profilin 1 antibody [EPR6304] (ab203146)

ab203146 staining Profilin 1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab203146 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Western blot - Alexa Fluor® 647 Anti-Profilin 1 antibody [EPR6304] (ab203146)

Clone EPR6304 has been knock-out validated in its unconjugated form. Please refer to ab124904 datasheet for experimental details.
Flow Cytometry - Alexa Fluor® 647 Anti-Profilin 1 antibody [EPR6304] (ab203146)

Overlay histogram showing HeLa cells stained with ab203146 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 35 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab203146, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 647 (ab199093) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a solid-state 25mW red diode laser (635 nm) and 675/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Alexa Fluor® 647 Anti-Profilin 1 antibody [EPR6304] (ab203146)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com