Anti-LIMK2 (phospho T505) antibody (ab38499)
Key features and details
- Rabbit polyclonal to LIMK2 (phospho T505)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LIMK2 (phospho T505) antibody
See all LIMK2 primary antibodies -
Description
Rabbit polyclonal to LIMK2 (phospho T505) -
Host species
Rabbit -
Specificity
This antibody is specific for LIMK2 only when phosphorylated at threonine 505, but may also cross react with LIMK1 phosphorylated at tyrosine 505 based on homology. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human LIMK2 (phospho T505).
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General notes
Previously labelled as LIM kinase 2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LIMK2 (phospho T505) antibody (ab38499)All lanes : Anti-LIMK2 (phospho T505) antibody (ab38499) at 1/500 dilution
Lane 1 : MCF7 cell extract (5-30ug of total protein) pre-incubated with phosphopeptide.
Lane 2 : MCF7 cell extract (5-30ug of total protein).
Secondary
All lanes : Alkaline Phosphatase Goat Anti-Rabbit IgG (H+L)
Predicted band size: 72 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LIMK2 (phospho T505) antibody (ab38499)ab38499 at a 1:50 dilution staining LIMK2 in Human breast carcinoma tissue using Immunohistochemistry, Paraffin Embedded Tissue.
Left image : untreated.
Right image : treated with phosphopeptide.
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Immunocytochemistry/ Immunofluorescence - Anti-LIMK2 (phospho T505) antibody (ab38499)ICC/IF image of ab38499 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38499, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
