Anti-Actin antibody [5J11] (ab190301)
![Anti-Actin antibody [5J11] (ab190301)](https://www.abcam.com/ps/products/190/ab190301/Images/ab190301-312747-anti-actin-antibody-5j11-western-blot.jpg "Anti-Actin antibody [5J11] (ab190301)")
Key features and details
- Mouse monoclonal [5J11] to Actin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Chicken, Human
- Isotype: IgG1
Overview
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Product name
Anti-Actin antibody [5J11]
See all Actin primary antibodies -
Description
Mouse monoclonal [5J11] to Actin -
Host species
Mouse -
Specificity
ab190301 binds to all six isotypes of mammalian actin. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Chicken, Human
Predicted to work with: Rabbit, Orangutan
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Immunogen
Full length native protein (purified) corresponding to Cow Actin aa 1 to the C-terminus. derived from bovine brain preparation.
Database link: P68138 -
Positive control
- WB: Rat and mouse brain lysate; NIH/3T3, HEK-293, HeLa and SH-SY5Ycell lysate. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 49.98% PBS, 49.98% Glycerol -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
5J11 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Actin antibody [5J11] (ab190301)All lanes : Anti-Actin antibody [5J11] (ab190301) at 1/1000 dilution
Lane 1 : Protein standard
Lane 2 : Rat brain lysate
Lane 3 : Mouse brain lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 7 : SH-SY5Y (human neuroblastoma cell line from bone marrow) cell lysate
Predicted band size: 42 kDaThe same blot was simultaneously probed with chicken pAb to UCHL-1, a marker of neuronal lineage cells (green).
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![Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [5J11] (ab190301)](https://www.abcam.com/ps/products/190/ab190301/Images/ab190301-225411-anti-actin-antibody-5j11-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [5J11] (ab190301)Immunofluorescent analysis of HeLa cells labeling Actin with ab190301 at 1/1000 dilution (green) and chicken polyclonal antibody to Vimentin (red). The blue stain reveals DNA in the nuclei of these cells.
