Anti-beta Actin antibody (ab227387)
Key features and details
- Rabbit polyclonal to beta Actin
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Drosophila melanogaster
- Isotype: IgG
Overview
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Product name
Anti-beta Actin antibody
See all beta Actin primary antibodies -
Description
Rabbit polyclonal to beta Actin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseRatIP HumanWB MouseRatHumanDrosophila melanogaster
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Immunogen
Recombinant fragment within Human beta Actin (internal sequence). The exact sequence is proprietary.
Database link: P60709 -
Positive control
- WB: HEK-293T, A431, HeLa, HepG2, Neuro-2a, C8D30, NIH/3T3, RAW 264.7, C2C12, PC-12 and Rat2 whole cell extracts; Mouse and rat liver tissue extracts; Drosophila melanogaster lysate. IP: HEK-293T whole cell extract. IHC-P: Mouse pancreas and cervix tissues; Rat liver tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)
Paraffin-embedded mouse pancreas tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody (ab227387)0.5% Triton X-100 followed by ice-cold methanol fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin (green) using ab227387 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Western blot - Anti-beta Actin antibody (ab227387)All lanes : Anti-beta Actin antibody (ab227387) at 1/20000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lane 5 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
Lane 6 : C8D30 whole cell extract
Lane 7 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
Lane 8 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
Lane 9 : C2C12 (mouse myoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)Paraffin-embedded rat liver tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-beta Actin antibody (ab227387)All lanes : Anti-beta Actin antibody (ab227387) at 1/20000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Western blot - Anti-beta Actin antibody (ab227387)All lanes : Anti-beta Actin antibody (ab227387) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 15 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 5 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 2 µg
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody (ab227387)Paraffin-embedded mouse cervix tissue stained for beta Actin using ab227387 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-beta Actin antibody (ab227387)Anti-beta Actin antibody (ab227387) at 1/10000 dilution + Drosophila melanogaster lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Western blot - Anti-beta Actin antibody (ab227387)All lanes : Anti-beta Actin antibody (ab227387) at 1/15000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with beta Actin shRNA, whole cell extract
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Immunoprecipitation - Anti-beta Actin antibody (ab227387)beta Actin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg of ab227387. Western blot was performed from the immunoprecipitate using ab227387. Anti-rabbit IgG was used as secondary antibody.
Lane 1: HEK-293T whole cell extract (Input).
Lane 2: Control IgG instead of ab227387 in HEK-293T whole cell extract.
Lane 3: ab227387 IP in HEK-293T whole cell extract.
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Western blot - Anti-beta Actin antibody (ab227387)All lanes : Anti-beta Actin antibody (ab227387) at 1/20000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 2 : Rat2 (rat fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Western blot - Anti-beta Actin antibody (ab227387)Anti-beta Actin antibody (ab227387) at 1/20000 dilution + Mouse liver tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
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Western blot - Anti-beta Actin antibody (ab227387)Anti-beta Actin antibody (ab227387) at 1/20000 dilution + Rat liver tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 42 kDa10% SDS-PAGE gel.
