Anti-beta Actin antibody [BLR057F] (ab252556)
![Anti-beta Actin antibody [BLR057F] (ab252556)](https://www.abcam.com/ps/products/252/ab252556/Images/ab252556-343366-antibetaActinantibodyBLR057F-immunohistochemistry-ovarian-carcinoma-human.jpg "Anti-beta Actin antibody [BLR057F] (ab252556)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR057F] to beta Actin
- Suitable for: WB, IHC-P, ICC
- Reacts with: Mouse, Human
Overview
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Product name
Anti-beta Actin antibody [BLR057F]
See all beta Actin primary antibodies -
Description
Rabbit monoclonal [BLR057F] to beta Actin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanWB MouseHuman
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Immunogen
Synthetic peptide within Human beta Actin aa 1-50. The exact sequence is proprietary. (NP_001092.1).
Database link: P60709 -
Positive control
- IHC-P: Human ovartian carcinoma tissue. ICC: HeLa cells. WB: U2-OS, A549, K562, HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 cell lysates.
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General notes
This product is sold under License from Bethyl Laboratories, Inc
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR057F -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [BLR057F] (ab252556)
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for beta Actin using ab252556 at 1/400 dilution in immunohistochemical analysis, followed by HRP conjugated. DAB staining.
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![Immunocytochemistry - Anti-beta Actin antibody [BLR057F] (ab252556)](https://www.abcam.com/ps/products/252/ab252556/Images/ab252556-343365-antibetaActinantibodyBLR057F-immunocytochemistry-HeLa-human.jpg)
Immunocytochemistry - Anti-beta Actin antibody [BLR057F] (ab252556)FFPE HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin using ab252556 at 1/200 dilution in ICC/IF, followed by HRP conjugated Goat Anti-Rabbit IgG. Substrate: DAB.
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![Western blot - Anti-beta Actin antibody [BLR057F] (ab252556)](https://www.abcam.com/ps/products/252/ab252556/Images/ab252556-343364-antibetaActinantibodyBLR057F-western-blot-human-mouse.jpg)
Western blot - Anti-beta Actin antibody [BLR057F] (ab252556)All lanes : Anti-beta Actin antibody [BLR057F] (ab252556) at 1/1000 dilution
Lane 1 : U2-OS (human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 7 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 8 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti-Rabbit IgG at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 1 secondLysates prepared using NETN lysis buffer.
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![Anti-beta Actin antibody [BLR057F] (ab252556)](https://www.abcam.com/ps/products/252/ab252556/Images/ab252556-4-benefits-of-recombinant-antibodies.png)
Anti-beta Actin antibody [BLR057F] (ab252556)
