Anti-alpha Adducin (phospho S726) antibody (ab53093)
Key features and details
- Rabbit polyclonal to alpha Adducin (phospho S726)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-alpha Adducin (phospho S726) antibody
See all alpha Adducin primary antibodies -
Description
Rabbit polyclonal to alpha Adducin (phospho S726) -
Host species
Rabbit -
Specificity
ab53093 detects endogenous levels of alpha Adducin only when phosphorylated at serine 726. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic phosphopeptide derived from human alpha Adducin around the phosphorylation site of serine 726 (TPSPFL).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab53093 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-alpha Adducin (phospho S726) antibody (ab53093)All lanes : Anti-alpha Adducin (phospho S726) antibody (ab53093) at 1/500 dilution
Lane 1 : HeLa cell extract treated with Forskolin (40nM, 30 mins) with phosphopeptide
Lane 2 : HeLa cell extract treated with Forskolin (40nM, 30 mins)
Predicted band size: 81 kDa
Observed band size: 81 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody (ab53093)labelled with ab53093, at 1/50 dilution, staining alpha Adducin in paraffin embedded human brain tissue sections by Immunohistochemistry. Tissues had either been treated with (right image) or without (left image) the phosphopeptide.
