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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin

Anti-alpha Adducin antibody [5D4H1] - N-terminal (ab204961)

Anti-alpha Adducin antibody [5D4H1] - N-terminal (ab204961)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [5D4H1] to alpha Adducin - N-terminal
  • Suitable for: IHC-P, WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

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Overview

  • Product name

    Anti-alpha Adducin antibody [5D4H1] - N-terminal
    See all alpha Adducin primary antibodies
  • Description

    Mouse monoclonal [5D4H1] to alpha Adducin - N-terminal
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
    Predicted to work with: Mouse, Rat, Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human alpha Adducin aa 1-200 (N terminal). Expressed in E. coli.
    Database link: P35611

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Human alpha Adducin (amino acids 1-193) recombinant protein. Lysate from HEK293 cells transfected with alpha Adducin (amino acids 1-193). Human cervical cancer and rectum cancer tissues.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    5D4H1
  • Isotype

    IgG1
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Actin
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKA
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calmodulin Pathway

Images

  • Western blot - Anti-alpha Adducin antibody [5D4H1] - N-terminal (ab204961)
    Western blot - Anti-alpha Adducin antibody [5D4H1] - N-terminal (ab204961)
    Anti-alpha Adducin antibody [5D4H1] - N-terminal (ab204961) at 1/500 dilution + Human alpha Adducin (amino acids 1-193) recombinant protein.

    Predicted band size: 81 kDa



    Note: the predicted band size of the full length protein is 81 kDa. The expected band size of the recombinant fragment used here is 48 kDa.

  • Western blot - Anti-alpha Adducin antibody [5D4H1] - N-terminal (ab204961)
    Western blot - Anti-alpha Adducin antibody [5D4H1] - N-terminal (ab204961)
    All lanes : Anti-alpha Adducin antibody [5D4H1] - N-terminal (ab204961) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Lysate from HEK293 cells transfected with alpha Adducin (amino acids 1-193).

    Predicted band size: 81 kDa



    Note: the predicted band size of the full length protein is 81 kDa. The expected band size of the recombinant fragment used here is 48 kDa.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin antibody [5D4H1] - N-terminal (ab204961)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin antibody [5D4H1] - N-terminal (ab204961)

    Immunohistochemical analysis of paraffin-embedded, DAB-stained Human cervical cancer tissue, labeling alpha Adducin using ab204961 at a 1/200 dilution

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin antibody [5D4H1] - N-terminal (ab204961)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin antibody [5D4H1] - N-terminal (ab204961)

    Immunohistochemical analysis of paraffin-embedded, DAB-stained Human rectum cancer tissue, labeling alpha Adducin using ab204961 at a 1/200 dilution

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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