Anti-alpha Adducin antibody (ab51130)
Key features and details
- Rabbit polyclonal to alpha Adducin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-alpha Adducin antibody
See all alpha Adducin primary antibodies -
Description
Rabbit polyclonal to alpha Adducin -
Host species
Rabbit -
Specificity
There are 2 isoforms of alpha adducin on the Uniprot protein database page for mouse. The antibody immunogen has high alignment to isoform 1: Isoform 1 80 kDa 100% Isoform 2 69 kDa 30% The aduccin protein is made up of 4 subunits, including the alpha beta and gamma subunits (according to the Uniprot page), Alignment of the immunogen with the mouse beta adducin: http://www.uniprot.org/uniprot/Q9QYB8 isoform 1 100% isoform 2 30% isoform 3 100% And with the mouse gamma adducin: http://www.uniprot.org/uniprot/Q9QYB5 isoform 1 92% Isoform 2 92% The antibody has not been specifically tested for detection of the other subunits, however according to the high alignments, the antibody is likely to detect several isoforms of these beta and gamma subunits. -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthesized non-phosphopeptide derived from human alpha Adducin around the phosphorylation site of serine 726 (T-P-SP-F-L).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-alpha Adducin antibody (ab51130)All lanes : Anti-alpha Adducin antibody (ab51130)
Lane 1 : Extracts from Hela cells treated
with Forskolin (40nM, 30min) with no peptide
Lane 2 : Extracts from Hela cells treated
with Forskolin (40nM, 30min) with with immunising peptide
Predicted band size: 81 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin antibody (ab51130)This image shows paraffin-embedded human brain tissue stained with ab51130 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue -
Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin antibody (ab51130)ICC/IF image of ab51130 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51130, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
