Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251)
![Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251)](https://www.abcam.com/ps/products/76/ab76251/Images/ab76251-91620-anti-alpha-adducin-phospho-s59-antibody-ep2175ay-western-blot.jpg "Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2175AY] to alpha Adducin (phospho S59)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-alpha Adducin (phospho S59) antibody [EP2175AY]
See all alpha Adducin primary antibodies -
Description
Rabbit monoclonal [EP2175AY] to alpha Adducin (phospho S59) -
Host species
Rabbit -
Specificity
ab76251 only detects alpha Adducin when phosphorylated at Serine 59. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Serine59 of human alpha Adducin
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Positive control
- HeLa cell lysates, untreated, or treated with calyculin A
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP2175AY -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251)All lanes : Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251) at 1/10000 dilution
Lane 1 : HeLa cell lysate (untreated)
Lane 2 : HeLa cell lysate, treated with calyculin A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP labelled IgG at 1/1000 dilution
Predicted band size: 81 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
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![Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251)](https://www.abcam.com/ps/products/76/ab76251/Images/ab76251-2-benefits-of-recombinant-antibodies.png)
Anti-alpha Adducin (phospho S59) antibody [EP2175AY] (ab76251)
