Anti-RNA polymerase beta antibody [EPR18704] (ab191598)
![Anti-RNA polymerase beta antibody [EPR18704] (ab191598)](https://www.abcam.com/ps/products/191/ab191598/Images/ab191598-246642-anti-rna-polymerase-beta-antibody-epr18704-western-blot.jpg "Anti-RNA polymerase beta antibody [EPR18704] (ab191598)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18704] to RNA polymerase beta
- Suitable for: IP, WB
- Reacts with: Escherichia coli
Overview
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Product name
Anti-RNA polymerase beta antibody [EPR18704]
See all RNA polymerase beta primary antibodies -
Description
Rabbit monoclonal [EPR18704] to RNA polymerase beta -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB & IP: E.coli whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18704 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RNA polymerase beta antibody [EPR18704] (ab191598)Anti-RNA polymerase beta antibody [EPR18704] (ab191598) at 1/2000 dilution + E.coli whole cell lysates at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 150 kDa
Observed band size: 150 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-RNA polymerase beta antibody [EPR18704] (ab191598)](https://www.abcam.com/ps/products/191/ab191598/Images/ab191598-246641-anti-rna-polymerase-beta-antibody-epr18704-immunoprecipitation.jpg)
Immunoprecipitation - Anti-RNA polymerase beta antibody [EPR18704] (ab191598)RNA polymerase beta was immunoprecipitated from 1mg of E.coli whole cell lysates with ab191598 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab191598 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: E.coli whole cell lysates 10ug (Input). Lane 2: ab191598 IP in E.coli whole cell lysates. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191598 in E.coli whole cell lysates.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
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![Anti-RNA polymerase beta antibody [EPR18704] (ab191598)](https://www.abcam.com/ps/products/191/ab191598/Images/ab191598-3-benefits-of-recombinant-antibodies.png)
Anti-RNA polymerase beta antibody [EPR18704] (ab191598)
