Anti-Aly/Ref antibody (ab246922)
Key features and details
- Rabbit polyclonal to Aly/Ref
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Aly/Ref antibody
See all Aly/Ref primary antibodies -
Description
Rabbit polyclonal to Aly/Ref -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Aly/Ref aa 100 to the C-terminus.
Database link: Q86V81 -
Positive control
- IHC-P: Human testis, liver, tonsil and placenta tissue. ICC/IF: U-2 OS cells. WB: Aly/Ref overexpression HEK-293T lysate. siRNA control transfected U-2 OS cells.
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General notes
This product was previously labelled as Aly
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Aly/Ref antibody (ab246922) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : Aly/Ref overexpression HEK-293T lysate
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PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling Aly/Ref using ab246922 at 4 µg/ml (green) in ICC/IF.
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Formalin-fixed, paraffin-embedded human testis tissue stained for Aly/Ref with ab246922 at a 1/50 dilution in immunohistochemical analysis.
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All lanes : Anti-Aly/Ref antibody (ab246922) at 0.4 µg/ml
Lane 1 : siRNA probe #1 transfected U-2 OS (Human bone osteosarcoma epithelial cell line) cells
Lane 2 : siRNA probe #2 transfected U-2 OS cells
Lane 3 : Control siRNA transfected U-2 OS cells
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for Aly/Ref with ab246922 at a 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human placenta tissue stained for Aly/Ref with ab246922 at a 1/50 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human liver tissue stained for Aly/Ref with ab246922 at a 1/50 dilution in immunohistochemical analysis.