Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-mSin3A antibody [EPR6780] (ab129087)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6780] to mSin3A
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-mSin3A antibody [EPR6780]
    See all mSin3A primary antibodies

  • Description

    Rabbit monoclonal [EPR6780] to mSin3A

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues on the C-terminus in Human mSin3A (UniProt ID: Q96ST3).

  • Positive control

    • HeLa, K562, 293T, PC12, RAW 264.7, and Mouse lung lysates, Human colon tissue

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-mSin3A antibody [EPR6780] (ab129087)
    Western blot - Anti-mSin3A antibody [EPR6780] (ab129087)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: mSin3A knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab129087 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab129087 was shown to recognize mSin3A when mSin3A knockout samples were used, along with additional cross-reactive bands. Wild-type and mSinA knockout samples were subjected to SDS-PAGE. ab129087 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody [EPR6780] (ab129087)
    Immunocytochemistry/ Immunofluorescence - Anti-mSin3A antibody [EPR6780] (ab129087)

    Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) labeling mSin3A with Purified ab129087 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody [EPR6780] (ab129087)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mSin3A antibody [EPR6780] (ab129087)

    ab129087, at a dilution of 1/50, staining mSin3A in paraffin-embedded Human colon tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-mSin3A antibody [EPR6780] (ab129087)
    Flow Cytometry - Anti-mSin3A antibody [EPR6780] (ab129087)

    Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling mSin3A with purified ab129087 at 1/250 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-mSin3A antibody [EPR6780] (ab129087)
    Western blot - Anti-mSin3A antibody [EPR6780] (ab129087)
    All lanes : Anti-mSin3A antibody [EPR6780] (ab129087) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : PC12 cell lysate
    Lane 5 : RAW 264.7 cell lysate
    Lane 6 : Mouse lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 145 kDa

  • OI-RD Scanning - Anti-mSin3A antibody [EPR6780] (ab129087)
    OI-RD Scanning - Anti-mSin3A antibody [EPR6780] (ab129087)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-mSin3A antibody [EPR6780] (ab129087)
    Anti-mSin3A antibody [EPR6780] (ab129087)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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