Anti-Tim 2 antibody [RMT2-14] - Chimeric (ab281910)
![Anti-Tim 2 antibody [RMT2-14] - Chimeric (ab281910)](https://www.abcam.com/ps/products/281/ab281910/Images/ab281910-1-anti-TIM2-antibody-immunohistochemistry-splenocytes-mouse.jpg "Anti-Tim 2 antibody [RMT2-14] - Chimeric (ab281910)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RMT2-14] to Tim 2 - Chimeric
- Suitable for: ICC
- Reacts with: Mouse
Overview
-
Product name
Anti-Tim 2 antibody [RMT2-14] - Chimeric
See all Tim 2 primary antibodies -
Description
Rabbit monoclonal [RMT2-14] to Tim 2 - Chimeric -
Host species
Rabbit -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Fusion protein corresponding to Mouse Tim 2 aa 1-250 (extracellular). Raised by immunising Sprague Dawley rats with TIM-2-Ig extracellular domain and the Fc portion of mouse IgG2a.
Database link: Q8R183 -
Positive control
- ICC: Mouse splenocytes cells.
-
General notes
ab281910 is the chimeric rabbit version of ab281909.
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
RMT2-14 -
Isotype
IgG -
Light chain type
lambda -
Research areas
Images
-
Immunocytochemistry - (ab281910)
Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix® coverslips stained with the chimeric ab281910 at 10 µg/ml for 1 hour, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml (green/top-left). Image showing membrane staining. The nuclear counter stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody. Mouse splenocytes were immobilized on Shi-fix® coverslips.