HRP Anti-Coilin antibody [IH10] (ab196715)
![HRP Anti-Coilin antibody [IH10] (ab196715)](https://www.abcam.com/ps/products/196/ab196715/Images/ab196715-256012-20151207ab196715ap2199645HuTestisnorm10ugml.jpg "HRP Anti-Coilin antibody [IH10] (ab196715)")
Key features and details
- HRP Mouse monoclonal [IH10] to Coilin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-Coilin antibody [IH10]
See all Coilin primary antibodies -
Description
HRP Mouse monoclonal [IH10] to Coilin -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Coilin.
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Positive control
- WB: HeLa, MCF7 and SHSY-5Y whole cell lysates. IHC-P: FFPE Human Testis Normal
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General notes
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
IH10 -
Isotype
IgG2b -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Coilin antibody [IH10] (ab196715)
IHC image of Collin staining in a section of formalin-fixed paraffin-embedded normal human testis*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab196715 at 1/50 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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![Western blot - HRP Anti-Coilin antibody [IH10] (ab196715)](https://www.abcam.com/ps/products/196/ab196715/Images/ab196715-241948-WBab1967151.jpg)
Western blot - HRP Anti-Coilin antibody [IH10] (ab196715)All lanes : HRP Anti-Coilin antibody [IH10] (ab196715) at 1/2000 dilution
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab196715 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
