Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD4 antibody [CAL4] - BSA and Azide free (ab251608)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAL4] to CD4 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, IHC-Fr, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-CD4 antibody [CAL4] - BSA and Azide free
    See all CD4 primary antibodies

  • Description

    Rabbit monoclonal [CAL4] to CD4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WB, IHC-Fr, IPmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Mouse CD4 aa 417-457. The exact sequence is proprietary.
    Database link: P06332

  • Positive control

    • IHC-P: Mouse and rat spleen tissue. WB: Mouse and rat thymus, spleen and lymph node lysate. IHC-Fr: Mouse spleen tissue. Flow Cyt (intra): Mouse splenocytes. IP: Mouse thymus lysate.

  • General notes

    ab251608 is the carrier-free version of ab237722.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [CAL4] - BSA and Azide free (ab251608)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [CAL4] - BSA and Azide free (ab251608)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD4 with ab237722 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse spleen. The section was incubated with ab237722 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237722).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [CAL4] - BSA and Azide free (ab251608)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [CAL4] - BSA and Azide free (ab251608)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD4 with ab237722 at 1/4000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat spleen. The section was incubated with ab237722 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237722).

  • Immunohistochemistry (Frozen sections) - Anti-CD4 antibody [CAL4] - BSA and Azide free (ab251608)
    Immunohistochemistry (Frozen sections) - Anti-CD4 antibody [CAL4] - BSA and Azide free (ab251608)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen tissue labeling CD4 with ab237722 at 1/100 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on T cell surface (PMID: 26994219) is observed. Counterstained with DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237722).

  • Flow Cytometry - Anti-CD4 antibody [CAL4] - BSA and Azide free (ab251608)
    Flow Cytometry - Anti-CD4 antibody [CAL4] - BSA and Azide free (ab251608)

    Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse splenocytes labeling CD4 with ab237722 at 1/40 (right panel) compared with a Rabbit monoclonal IgG (ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD4 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min and intracellularly stained with rabbit IgG (Left) or ab237722 (Right). They recognize the same cell population.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237722).

  • Immunoprecipitation - Anti-CD4 antibody [CAL4] - BSA and Azide free (ab251608)
    Immunoprecipitation - Anti-CD4 antibody [CAL4] - BSA and Azide free (ab251608)

    CD4 was immunoprecipitated from 0.35 mg mouse thymus lysate with ab237722 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237722 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used at 1/5000 dilution.
    Lane 1: Mouse thymus lysate 10 µg (Input).
    Lane 2: ab237722 IP in mouse thymus lysate .
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237722 in mouse thymus lysate .
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    102kDa band represents CD4 dimer based on literature. (PMID: 20739067).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237722).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [CAL4] - BSA and Azide free (ab251608)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [CAL4] - BSA and Azide free (ab251608)

    Formalin-fixed, paraffin-embedded mouse spleen tissue stained for CD4 using ab237722 at 0.5 µg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237722).

  • Anti-CD4 antibody [CAL4] - BSA and Azide free (ab251608)
    Anti-CD4 antibody [CAL4] - BSA and Azide free (ab251608)

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