Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Immunology Adaptive Immunity T Cells CD

Anti-CD4 antibody [EPR19533] (ab183686)

Price and availability

294 835 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-CD4 antibody [EPR19533] (ab183686)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19533] to CD4
  • Suitable for: WB
  • Reacts with: Mouse, Rat

You may also be interested in

Anti-Plasma Cell antibody [LIV3G11] (ab44876)
Product image
Anti-CD4 antibody [SP35] (ab213215)
Product image
PE Anti-CD109 antibody [W7C5] (ab275953)
Recombinant Human CD272/BTLA protein (ab152051)

Overview

  • Product name

    Anti-CD4 antibody [EPR19533]
    See all CD4 primary antibodies
  • Description

    Rabbit monoclonal [EPR19533] to CD4
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse thymus and spleen lysates; Rat thymus and spleen lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19533
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related

Images

  • Western blot - Anti-CD4 antibody [EPR19533] (ab183686)
    Western blot - Anti-CD4 antibody [EPR19533] (ab183686)
    All lanes : Anti-CD4 antibody [EPR19533] (ab183686) at 1/1000 dilution

    Lane 1 : Mouse thymus lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Rat liver lysate
    Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 8 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 9 : Rat thymus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1,2,3,4,5,6,7 and 8: 3 minutes; Lane 9: 5 seconds.

    No band observed in lane 2 to lane 8 in our testing condition but CD4 should be specifically expressed in a few T cells.

  • Western blot - Anti-CD4 antibody [EPR19533] (ab183686)
    Western blot - Anti-CD4 antibody [EPR19533] (ab183686)
    All lanes : Anti-CD4 antibody [EPR19533] (ab183686) at 1/1000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time:3 minutes

  • Anti-CD4 antibody [EPR19533] (ab183686)
    Anti-CD4 antibody [EPR19533] (ab183686)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CD4 antibody [EPR19533] (ab183686)

  •  
  • Product image

    PE/Cy7® Anti-CD4 antibody [MEM241] (ab233660)

    Applications: Flow Cyt

  •  
  • Product image

    Anti-CD4 antibody [CAL4] (ab237722)

    Applications: Flow Cyt, IHC-Fr, IHC-P, IP, WB

  •  
  • Product image

    Anti-CD4 antibody [EPR6855] - BSA and Azide free (ab181724)

    Applications: Flow Cyt, ICC, IHC-P, Mass Cytometry, WB

  •  
  • Product image

    Anti-CD4 antibody [EPR6855] (ab133616)

    Applications: Flow Cyt, ICC, IHC-P, WB

  •  
  • Product image

    Alexa Fluor® 594 Anti-CD4 antibody [EPR6855] (ab277931)

    Applications: IHC-P

  •  
  • Product image

    Alexa Fluor® 647 Anti-CD4 antibody [EPR6855] (ab196147)

    Applications: ICC/IF

  •  
  • Product image

    Anti-CD4 antibody [EPR19514] (ab183685)

    Applications: IHC-Fr, IHC-P, IP, WB

  •  
  • Product image

    Anti-CD4 antibody [SP35] - BSA and Azide free (ab238798)

    Applications: Flow Cyt, IHC-P

  •  
  • Product image

    Anti-CD4 antibody [EPR19514] - Low endotoxin, Azide free (ab221775)

    Applications: IHC-Fr, IHC-P, IP, WB

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.