Recombinant cynomolgus monkey CD86 protein (Fc Chimera Active) (ab220540)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose, Sodium chloride
Lyophilized from 0.22 µm filtered solution.
5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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Functional Studies - Recombinant cynomolgus monkey CD86 protein (Fc Chimera Active) (ab220540)
Immobilized Cynomolgus CTLA-4, His Tag at 2 μg/mL (100 μL/well) can bind ab220540 with a linear range of 0.039-1.25 μg/mL.
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SDS-PAGE - Recombinant cynomolgus monkey CD86 protein (Fc Chimera Active) (ab220540)SDS-PAGE analysis ofa b220540 under reducing conditions, stained overnight with Coomassie Blue. As a result of glycosylation, the reduced protein migrates as 80-110 kDa.
