Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 10, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20122] to CD4
Suitable for: WB, Flow Cyt, IP
Reacts with: Mouse

Product name

Anti-CD4 antibody [EPR20122]
See all CD4 primary antibodies
Description

Rabbit monoclonal [EPR20122] to CD4
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
IP	Mouse
WB	Mouse

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse thymus lysate. Flow Cyt: Mouse thymocytes. IP: Mouse thymus lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20122
Isotype

IgG
Research areas

Flow Cytometry - Anti-CD4 antibody [EPR20122] (ab207755)

Flow cytometric analysis of Mouse thymocytes labeling CD4 with ab207755 at 1/60 dilution (red), compared with ab172730 (Rabbit IgG, monoclonal [EPR25A] - Isotype Control) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-CD4 antibody [EPR20122] (ab207755)

Anti-CD4 antibody [EPR20122] (ab207755) at 1/5000 dilution + Mouse thymus at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 51 kDa

Exposure time: 5 seconds

Blocking and Diluting buffer and concentration: 5% NFDM/TBST.
Immunoprecipitation - Anti-CD4 antibody [EPR20122] (ab207755)

CD4 was immunoprecipitated from 0.35 mg of Mouse thymus lysate with ab207755 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab207755 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Mouse thymus lysate, 10 μg (Input).

Lane 2: ab207755 IP in Mouse thymus lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207755 in Mouse thymus lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds.
Anti-CD4 antibody [EPR20122] (ab207755)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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