Recombinant human CD22 protein (Active) (Biotin) (ab252370)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Avi tag C-Terminus, Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Preparation and Storage
- B cell receptor CD22 precursor
- B lymphocyte cell adhesion molecule
- B-cell receptor CD22
Images
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Functional Studies - Recombinant human CD22 protein (Active) (Biotin) (ab252370)
293 cells were transfected with anti-CD22-scFv and RFP tag. 2e5 of the cells were first stained with B. Biotinylated Human Siglec-2, Fc,Avitag and C. Biotinylated Protein Control, followed by FITC Streptavidin. A. Non-transfected 293 cells and C. Biotinylated Protein Control were used as negative control. RFP was used to evaluate CAR (anti-CD22-scFv) expression and FITC was used to evaluate the binding activity of Biotinylated Human Siglec-2, Fc,Avitag .
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SDS-PAGE - Recombinant human CD22 protein (Active) (Biotin) (ab252370)SDS-PAGE - Recombinant human CD22 protein (Active) (Biotin) (ab252370).
The gel was stained overnight with Coomassie Blue.
The protein migrates as 115-120 kDa under reducing conditions due to glycosylation.
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Functional Studies - Recombinant human CD22 protein (Active) (Biotin) (ab252370)Immobilized Anti-Human CD22 MAb at 0.5 μg/mL (100 μL/well) can bind ab252370 with a linear range of 1-16 ng/mL.
