Human D-Dimer ELISA Kit, Fluorescent (ab229437)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.496 ng/ml
- Range: 2.344 ng/ml - 1200 ng/ml
- Sample type: Cell culture media, Cit plasma, Hep Plasma, Serum, Urine
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human D-Dimer ELISA Kit, Fluorescent
See all D-Dimer kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.4% Inter-assay Sample n Mean SD CV% Serum 3 4.3% -
Sample type
Urine, Serum, Cell culture media, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.496 ng/ml -
Range
2.344 ng/ml - 1200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 101 99% - 103% Serum 107 96% - 124% Cell culture media 83 81% - 84% Hep Plasma 83 99% - 103% Cit plasma 98 95% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
D-Dimer in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of D-Dimer protein in human serum, plasma, urine, and cell culture supernatants.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
Plasma collected with EDTA is not compatible with this kit.
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Notes
D-dimer is a protein formed by the cross-linking of two D fragments of the fibrin protein. D-dimer is one of several fibrin degradation products (FDPs) formed by the degradation of a blood clot by fibrinolysis. Its measurement is used to diagnose the blood disorder disseminated intravascular coagulation and in the diagnosis of thrombosis.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X Stoplight Red Substrate 1 x 120µl 10X Human D-Dimer Capture Antibody 1 x 600µl 10X Human D-Dimer Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl Antibody Diluent 4BI 1 x 6ml Human D-Dimer Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit Stoplight Red Substrate Buffer 1 x 12ml -
Research areas
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Relevance
A fibrin degradation fragment or product that is produced by the action of plasmin on fibrin in the clot dissolution process. -
Alternative names
- D Dimer
Images
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Catchpoint ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human D-Dimer standard curve in Sample Diluent NS.The D-Dimer standard curve was prepared as described in Section 10. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native D-Dimer in human serum, plasma (citrate), and plasma (heparin) samples.The concentrations of D-Dimer were measured in duplicates, interpolated from the D-Dimer standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (citrate) 10%, and plasma (heparin) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean D-Dimer concentration was determined to be 13.13 µg/mL in serum, 2.79 µg/mL in plasma (citrate), and 0.66 µg/ml (656.15 ng/mL) in plasma (heparin).
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Serum from ten individual healthy human pooled sex donors was measured in duplicate. -
Sandwich ELISA - Human D-Dimer ELISA Kit, Fluorescent (ab229437)To learn more about the advantages of recombinant antibodies see here.