Human TPFI2 ELISA Kit (ab275908)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 16 pg/ml
- Range: 56.25 pg/ml - 3600 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human TPFI2 ELISA Kit
See all TFPI2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 8 3.6% Inter-assay Sample n Mean SD CV% Plasma 3 7% -
Sample type
Cell culture supernatant, Serum, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
16 pg/ml -
Range
56.25 pg/ml - 3600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 95 88% - 101% Serum 103 96% - 113% Cit plasma 96 88% - 103% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human TFPI2 ELISA kit (ab275908) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human TFPI2 protein in human serum, plasma-citrate, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human TFPI2 with 16 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
TFPI2, Tissue factor pathway inhibitor 2 also known as placental protein 5 (PP5), is a serine protease inhibitor that plays a key role in plasmin-mediated remodeling of the matrix by inhibiting trypsin and plasmin. TFPI2 is involved in matrix homeostasis, fetal development, and preventing tumor metastasis through invasion. Hypermethylation of this protein is associated with many cancer types, including, colorectal, pancreatic, and cervical cancers. The immunogen used to create the antibodies for this kit represents the mature form of the protein. The protein homologs in mouse in rat share little sequence homology, and as such, are not expected to cross-react with this kit.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human TFPI2 Capture Antibody 1 x 600µl 10X Human TFPI2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Human TFPI2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May play a role in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. Has no effect on thrombin. -
Tissue specificity
Umbilical vein endothelial cells, liver, placenta, heart, pancreas, and maternal serum at advanced pregnancy. -
Sequence similarities
Contains 3 BPTI/Kunitz inhibitor domains. -
Domain
This inhibitor contains three inhibitory domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Placental protein 5
- PP5
- REF1
see all -
Database links
- Entrez Gene: 7980 Human
- Omim: 600033 Human
- SwissProt: P48307 Human
- Unigene: 438231 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human TFPI2 standard curve in Sample Diluent NS.The TFPI2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native TFPI2 in human serum, plasma and cell culture supernatant samples.The concentrations of TFPI2 were measured in duplicates, interpolated from the TFPI2 standard curves and corrected for sample dilution. Undiluted samples are as follows: pregnant serum 1:100, pregnant plasma (citrate) 1:100, and HeLa cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFPI2 concentration was determined to be 294.3 ng/mL in pregnant serum, 347 ng/mL in pregnant plasma (citrate), and 1.6 ng/mL in HeLa cell culture supernatant.
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Sandwich ELISA - Human TPFI2 ELISA Kit (ab275908)To learn more about the advantages of recombinant antibodies see here.
