Factor XII / XIIa Assay Kit (ab241041)
Key features and details
- Detection method: Colorimetric
- Sample type: Plasma
Overview
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Product name
Factor XII / XIIa Assay Kit -
Detection method
Colorimetric -
Sample type
Plasma -
Species reactivity
Reacts with: Mammals -
Product overview
The Factor XIIa Assay Kit (ab241041) utilizes the ability of factor XIIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD405 nm). The kit is easy-to-use and can detect Factor XIIa (as low as 1 mPEU) from plasma samples. This kit also measures Factor XII. In the sample preparation, the FXII is converted to FXIIa by the FXII activator.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests FXIIa Activator 1 x 1ml FXIIa Assay Buffer 1 x 25ml FXIIa Inhibitor 1 x 0.1ml FXIIa Substrate 1 x 0.1ml Human Factor XIIa 1 vial Human Factor XIIa Supplement 1 x 10µl pNA Standard (0.1 M) 1 x 20µl -
Research areas
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Function
Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa. -
Involvement in disease
Defects in F12 are the cause of factor XII deficiency (FA12D) [MIM:234000]; also known as Hageman factor deficiency. This trait is an asymptomatic anomaly of in vitro blood coagulation. Its diagnosis is based on finding a low plasma activity of the factor in coagulating assays. It is usually only accidentally discovered through pre-operative blood tests. F12 deficiency is divided into two categories, a cross-reacting material (CRM)-negative group (negative F12 antigen detection) and a CRM-positive group (positive F12 antigen detection).
Defects in F12 are the cause of hereditary angioedema type 3 (HAE3) [MIM:610618]; also known as estrogen-related HAE or hereditary angioneurotic edema with normal C1 inhibitor concentration and function. HAE is characterized by episodic local subcutaneous edema, and submucosal edema involving the upper respiratory and gastrointestinal tracts. HAE3 occurs exclusively in women and is precipitated or worsened by high estrogen levels (e.g., during pregnancy or treatment with oral contraceptives). It differs from HAE types 1 and 2 in that both concentration and function of C1 inhibitor are normal. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 fibronectin type-I domain.
Contains 1 fibronectin type-II domain.
Contains 1 kringle domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsFactor XII is activated by kallikrein in alpha-factor XIIa, which is then further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of the NH2-terminal heavy chain (Coagulation factor XIIa heavy chain) and the COOH-terminal light chain (Coagulation factor XIIa light chain), connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, a light chain (Beta-factor XIIa part 2), corresponding to the COOH-terminal light chain (Coagulation factor XIIa light chain) and a nonapeptide (Beta-factor XIIa part 1).
O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Coagulation factor XII
- coagulation factor XIIa heavy chain
- Coagulation factor XIIa light chain
see all
Images
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Kinetic progressive curves.Kinetic progressive curves for different amounts of FXIIa Enzyme
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Kinetic progressive curves.Kinetic progressive curves for different amounts of Activated Plasma Samples are shown. -
Standard curve for pNA.Standard curve for pNA. -
PK activity.Standard curve for pNA (n = 3) was used to estimate FXIIa activity in Normal Pooled Human Plasma and FXII-deficient Human Plasma (n = 3) (D). Assays were performed according to the kit protocol.
