Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17272-21] to PAI1 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free
    See all PAI1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17272-21] to PAI1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Hepa1-6 cells. WB: A549 and HUVEC cell lysates; Mouse placenta tissue lysate, rat placenta tissue lysate, rat lung tissue lysate.

  • General notes

    Ab226864 is the carrier-free version of ab182973. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab226864 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)
    Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)
    All lanes : Anti-PAI1 antibody [EPR17272-21] (ab182973) at 1/1000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : SERPINE1 knockout A549 cell lysate
    Lane 3 : HUVEC cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 48 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab182973).

    Lanes 1 - 3: Merged signal (red and green). Green - ab182973 observed at 48 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab182973 was shown to react with PAI1 in wild-type A549 cells in Western blot. The band observed in the edited lysate lane above 45 kDa is likely to represent SERPINE1 with an insertion. This has not been investigated further. Wild-type A549 and SERPINE1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab182973 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)
    Immunocytochemistry/ Immunofluorescence - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling PAI1 with ab182973 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cells.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).


    -ve control: PBS only, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182973).

  • Flow Cytometry - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)
    Flow Cytometry - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methonol-permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling PAI1 with ab182973 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182973).

  • Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)
    Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)
    All lanes : Anti-PAI1 antibody [EPR17272-21] (ab182973) at 1/1000 dilution

    Lane 1 : Rat placenta tissue lysate
    Lane 2 : Rat lung tissue lysate
    Lane 3 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 20 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    The expression level of mouse and rat PAI1 may be low in normal liver tissue (PMID: 21898503). This antibody detects no bands in rat liver.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182973).

  • Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)
    Western blot - Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)
    All lanes : Anti-PAI1 antibody [EPR17272-21] (ab182973) at 1/1000 dilution

    Lane 1 : Mouse placenta tissue lysate
    Lane 2 : Mouse liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    The expression level of mouse and rat PAI1 may be low in normal liver tissue (PMID: 21898503). This antibody detects a 37kDa extra band without BOI in mouse liver.

    Exposure time:

    Lane 1 : 3.25 seconds
    Lane 2 : 180 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab182973).

  • Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)
    Anti-PAI1 antibody [EPR17272-21] - BSA and Azide free (ab226864)

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