Anti-IL-21 antibody (ab5978)
Key features and details
- Rabbit polyclonal to IL-21
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-21 antibody
See all IL-21 primary antibodies -
Description
Rabbit polyclonal to IL-21 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Dog, Pig
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Immunogen
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Positive control
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General notes
A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IL-21 antibody (ab5978)
All lanes : Anti-IL-21 antibody (ab5978) at 1 µg/mL, 1h incubation at RT in 5% NFM/TBST.
Loading: 15 μg of lysates per lane.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution.
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Western blot - Anti-IL-21 antibody (ab5978)Western blot analysis of IL-21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with ab5978 at 1µ g /ml. Western blot analysis of IL-21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with ab5978 at 1 µg /ml. -
Western blot - Anti-IL-21 antibody (ab5978) -
Immunocytochemistry/ Immunofluorescence - Anti-IL-21 antibody (ab5978)ab5978 at 2ug/ml staining of IL21 in K562 cells.
