Anti-Interferon gamma antibody [EP1109Y] (ab52746)
![Anti-Interferon gamma antibody [EP1109Y] (ab52746)](https://www.abcam.com/ps/products/52/ab52746/Images/ab52746-261570-anti-interferon-gamma-antibody-ep1109y-western-blot.jpg "Anti-Interferon gamma antibody [EP1109Y] (ab52746)")
Key features and details
- Rabbit monoclonal [EP1109Y] to Interferon gamma
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Interferon gamma antibody [EP1109Y]
See all Interferon gamma primary antibodies -
Description
Rabbit monoclonal [EP1109Y] to Interferon gamma -
Host species
Rabbit -
Specificity
This antibody reacts with Interferon gamma. -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide within Human Interferon gamma aa 100-200. The exact sequence is proprietary.
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Positive control
- Human Interferon gamma recombinant protein
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.15% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1109Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Interferon gamma antibody [EP1109Y] (ab52746)Anti-Interferon gamma antibody [EP1109Y] (ab52746) at 1/1000 dilution + Human Interferon gamma recombinant protein at 0.015 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 19 kDaBlocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-Interferon gamma antibody [EP1109Y] (ab52746)](https://www.abcam.com/ps/products/52/ab52746/Images/ab52746-65169-anti-interferon-gamma-antibody-ep1109y-western-blot.jpg)
Western blot - Anti-Interferon gamma antibody [EP1109Y] (ab52746)Anti-Interferon gamma antibody [EP1109Y] (ab52746) at 1/2000 dilution + recombinant protein at 0.01 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 19 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
