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Epigenetics and Nuclear Signaling Histones H3 Methylated

Anti-Histone H4 (tri methyl K20) antibody (ab227884)

Anti-Histone H4 (tri methyl K20) antibody (ab227884)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Histone H4 (tri methyl K20)
  • Suitable for: WB, IHC-P, Dot blot, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Histone H4 (tri methyl K20) antibody
    See all Histone H4 primary antibodies
  • Description

    Rabbit polyclonal to Histone H4 (tri methyl K20)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Dot blot, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Xenopus laevis
  • Immunogen

    Synthetic peptide within Human Histone H4 (N terminal) (tri methyl K20). The exact sequence is proprietary. Conjugated to a carrier protein.
    Database link: P62805

  • Positive control

    • WB: HeLa whole cell and nuclear lysates. IHC-P: Mouse duodenum, liver and muscle tissues; Rat fore brain tissue. ICC/IF: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H4
    • Methylated

Images

  • Western blot - Anti-Histone H4 (tri methyl K20) antibody (ab227884)
    Western blot - Anti-Histone H4 (tri methyl K20) antibody (ab227884)
    All lanes : Anti-Histone H4 (tri methyl K20) antibody (ab227884) at 1/2000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 11 kDa



    15 % SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody (ab227884)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody (ab227884)

    Paraffin-embedded mouse duodenum tissue stained for Histone H4 (tri methyl K20) using ab227884 at 1/500 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (tri methyl K20) antibody (ab227884)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H4 (tri methyl K20) antibody (ab227884)

    HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (tri methyl K20) (green) using ab227884 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Red: alpha Tubulin stained with an alpha Tubulin antibody at 1/500 dilution..
    Blue: Hoechst 33342 staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody (ab227884)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody (ab227884)

    Paraffin-embedded mouse liver tissue stained for Histone H4 (tri methyl K20) using ab227884 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody (ab227884)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody (ab227884)

    Paraffin-embedded mouse muscle tissue stained for Histone H4 (tri methyl K20) using ab227884 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody (ab227884)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (tri methyl K20) antibody (ab227884)

    Paraffin-embedded rat fore brain tissue stained for Histone H4 (tri methyl K20) using ab227884 at 1/500 dilution in immunohistochemical analysis.

  • Dot Blot - Anti-Histone H4 (tri methyl K20) antibody (ab227884)
    Dot Blot - Anti-Histone H4 (tri methyl K20) antibody (ab227884)

    Dot Blot analysis of anti-Histone H4 (tri methyl Lys20) antibody with peptide samples.

    Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with ab227884 at 1/5000 dilution.

    A: Peptide samples of H4K20 un
    B: Peptide samples of H4K20me1
    C: Peptide samples of H4K20me2
    D: Peptide samples of H4K20me3

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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