Anti-Histone H4 (tri methyl K20) antibody (ab227884)
Key features and details
- Rabbit polyclonal to Histone H4 (tri methyl K20)
- Suitable for: WB, IHC-P, Dot blot, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H4 (tri methyl K20) antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (tri methyl K20) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Dot blot, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Xenopus laevis -
Immunogen
Synthetic peptide within Human Histone H4 (N terminal) (tri methyl K20). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: P62805 -
Positive control
- WB: HeLa whole cell and nuclear lysates. IHC-P: Mouse duodenum, liver and muscle tissues; Rat fore brain tissue. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Histone H4 (tri methyl K20) antibody (ab227884) at 1/2000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 11 kDa15 % SDS-PAGE gel.
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Paraffin-embedded mouse duodenum tissue stained for Histone H4 (tri methyl K20) using ab227884 at 1/500 dilution in immunohistochemical analysis.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (tri methyl K20) (green) using ab227884 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Red: alpha Tubulin stained with an alpha Tubulin antibody at 1/500 dilution..
Blue: Hoechst 33342 staining. -
Paraffin-embedded mouse liver tissue stained for Histone H4 (tri methyl K20) using ab227884 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse muscle tissue stained for Histone H4 (tri methyl K20) using ab227884 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded rat fore brain tissue stained for Histone H4 (tri methyl K20) using ab227884 at 1/500 dilution in immunohistochemical analysis.
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Dot Blot analysis of anti-Histone H4 (tri methyl Lys20) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with ab227884 at 1/5000 dilution.
A: Peptide samples of H4K20 un
B: Peptide samples of H4K20me1
C: Peptide samples of H4K20me2
D: Peptide samples of H4K20me3