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Epigenetics and Nuclear Signaling Histones H3 Methylated

Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Histone H3 (asymmetric di methyl R2)
  • Suitable for: WB, IHC-P, ICC/IF, Dot blot
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Histone H3 (asymmetric di methyl R2) antibody
    See all Histone H3 primary antibodies
  • Description

    Rabbit polyclonal to Histone H3 (asymmetric di methyl R2)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Dot blotmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Synthetic peptide within Human Histone H3 (asymmetric di methyl R2). The exact sequence is proprietary. A synthetic methylated peptide corresponding to residues surrounding R2 of Human Histone H3.
    Database link: Q16695

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Methylated

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)

    Paraffin-embedded human colon tissue stained for Histone H3 (asymmetric di methyl R2) using ab194706 at 1/100 dilution in immunohistochemical analysis. 

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)

    Immunofluorescence staining of HeLa cells stained for Histone H3 (asymmetric di methyl R2) with ab194706 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

  • Western blot - Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)
    Western blot - Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)
    All lanes : Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : H3 protein

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP Goat Anti-Rabbit IgG (H+L)

    Developed using the ECL technique.

    Predicted band size: 16 kDa


    Exposure time: 10 seconds


    Blocking buffer: 3% nonfat dry milk in TBST.

  • Dot Blot - Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)
    Dot Blot - Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)

    Dot-blot analysis of methylation peptides using ab194706.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)

    Paraffin-embedded mouse brain tissue stained for Histone H3 (asymmetric di methyl R2) using ab194706 at 1/100 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)

    Immunofluorescence staining of NIH/3T3 stained for Histone H3 (asymmetric di methyl R2) with ab194706 at 1/100 dilution. Nuclei are labeled with DAPI (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)

    Paraffin-embedded rat brain tissue stained for Histone H3 (asymmetric di methyl R2) using ab194706 at 1/100 dilution in immunohistochemical analysis. 

  • Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)
    Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (asymmetric di methyl R2) antibody (ab194706)

    Immunofluorescence staining of 293T cells stained for Histone H3 (asymmetric di methyl R2) with ab194706. Nuclei are labeled with DAPI (Blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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