HRP Anti-STAT1 alpha antibody [EPYR2154] (ab193891)
![HRP Anti-STAT1 alpha antibody [EPYR2154] (ab193891)](https://www.abcam.com/ps/products/193/ab193891/Images/ab193891-310941-anti-stat1-alpha-antibody-epyr2154-hrp-western-blot.jpg "HRP Anti-STAT1 alpha antibody [EPYR2154] (ab193891)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPYR2154] to STAT1 alpha
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
- Conjugation: HRP
Overview
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Product name
HRP Anti-STAT1 alpha antibody [EPYR2154]
See all STAT1 alpha primary antibodies -
Description
HRP Rabbit monoclonal [EPYR2154] to STAT1 alpha -
Host species
Rabbit -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HEK293, NIH-3T3 and A431 cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPYR2154 -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-STAT1 alpha antibody [EPYR2154] (ab193891)All lanes : HRP Anti-STAT1 alpha antibody [EPYR2154] (ab193891) at 1/2000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : STAT1 knockout HAP1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 87 kDa
Observed band size: 91 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesab193891 was shown to specifically react with STAT1 alpha in wild-type HAP1 cells as signal was lost in STAT1 knockout cells. Wild-type and STAT1 knockout samples were subjected to SDS-PAGE. Ab193891 and ab184095 (Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control (Alexa Fluor® 680) loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. The loading control was imaged using the Licor Odyssey CLx prior to blots being developed with ECL technique.
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![Western blot - HRP Anti-STAT1 alpha antibody [EPYR2154] (ab193891)](https://www.abcam.com/ps/products/193/ab193891/Images/ab193891-234102-anti-stat1-alpha-antibody-epyr2154-hrp-western-blot.jpg)
Western blot - HRP Anti-STAT1 alpha antibody [EPYR2154] (ab193891)All lanes : HRP Anti-STAT1 alpha antibody [EPYR2154] (ab193891) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035) at 10 µg
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 20 µg
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 91 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab193891 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![HRP Anti-STAT1 alpha antibody [EPYR2154] (ab193891)](https://www.abcam.com/ps/products/193/ab193891/Images/ab193891-3-benefits-of-recombinant-antibodies.png)
HRP Anti-STAT1 alpha antibody [EPYR2154] (ab193891)
