Anti-CD34 antibody - Hematopoietic Stem Cell Marker (ab150060)
Key features and details
- Rabbit polyclonal to CD34 - Hematopoietic Stem Cell Marker
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD34 antibody - Hematopoietic Stem Cell Marker
See all CD34 primary antibodies -
Description
Rabbit polyclonal to CD34 - Hematopoietic Stem Cell Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig, Chimpanzee, Macaque monkey, Chinese hamster
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Immunogen
Synthetic peptide corresponding to Human CD34 aa 300 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab140811) -
Positive control
- This antibody gave a positive signal in Human Lung and Human Fetal Lung tissue lysates, as well as TF-1 whole cell lysate. IHC-P: Human lung FFPE tissue sections
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab150060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 90, 105 kDa (predicted molecular weight: 41 kDa). IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Target
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Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
Tissue specificity
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
Sequence similarities
Belongs to the CD34 family. -
Developmental stage
On early hematopoietic progenitor cells. -
Post-translational
modificationsHighly glycosylated.
Phosphorylated on serine residues by PKC. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 947 Human
- Entrez Gene: 12490 Mouse
- Entrez Gene: 305081 Rat
- Omim: 142230 Human
- SwissProt: P28906 Human
- SwissProt: Q64314 Mouse
- Unigene: 374990 Human
- Unigene: 29798 Mouse
see all -
Alternative names
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody - Hematopoietic Stem Cell Marker (ab150060)IHC image of CD34 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab150060, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-CD34 antibody - Hematopoietic Stem Cell Marker (ab150060)Anti-CD34 antibody - Hematopoietic Stem Cell Marker (ab150060) at 1 µg/ml + TF-1 (Human erythroleukaemic cell line) Whole Cell Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes -
Western blot - Anti-CD34 antibody - Hematopoietic Stem Cell Marker (ab150060)All lanes : Anti-CD34 antibody - Hematopoietic Stem Cell Marker (ab150060) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa, 43 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Protocols
Datasheets and documents
References (3)
ab150060 has been referenced in 3 publications.
- Veron AD et al. Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera. BMC Vet Res 14:17 (2018). PubMed: 29343270
- Nakamichi M et al. Basic Fibroblast Growth Factor Induces Angiogenic Properties of Fibrocytes to Stimulate Vascular Formation during Wound Healing. Am J Pathol 186:3203-3216 (2016). PubMed: 27773739
- Tian D et al. Expression of brain-specific angiogenesis inhibitor-1 and association with p53, microvessel density and vascular endothelial growth factor in the tissue of human bladder transitional cell carcinoma. Mol Med Rep 12:4522-4529 (2015). PubMed: 26129954
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody - Hematopoietic Stem Cell Marker (ab150060)IHC image of CD34 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab150060, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-CD34 antibody - Hematopoietic Stem Cell Marker (ab150060)Anti-CD34 antibody - Hematopoietic Stem Cell Marker (ab150060) at 1 µg/ml + TF-1 (Human erythroleukaemic cell line) Whole Cell Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
-
Western blot - Anti-CD34 antibody - Hematopoietic Stem Cell Marker (ab150060)All lanes : Anti-CD34 antibody - Hematopoietic Stem Cell Marker (ab150060) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Lung (Human) Whole Cell Lysate - fetal normal tissue (ab30282)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa, 43 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
