Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPYR2154] to STAT1 alpha
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-STAT1 alpha antibody [EPYR2154]
    See all STAT1 alpha primary antibodies

  • Description

    Rabbit monoclonal [EPYR2154] to STAT1 alpha

  • Host species

    Rabbit

  • Specificity

    Based on Blast results using the immunogen sequence, this antibody should recognise the alpha but not the beta form (only 30% homology) of Stat1.

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    corresponding to Human STAT1 alpha aa 700 to the C-terminus.
    Database link: P42224

  • Positive control

    • HeLa, HEK293, NIH-3T3 and A431 cell lysates, mouse brain tissue lysate

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)
    Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: STAT1 alpha knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: A431 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab92506 (unpurified) observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab92506 was shown to specifically react with STAT1 alpha when STAT1 alpha knockout samples were used. Wild-type and STAT1 alpha knockout samples were subjected to SDS-PAGE. Ab92506 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)
    Immunoprecipitation - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

    ab92506 (purified) at 1/20 dilution (0.5ug) immunoprecipitating STAT1 alpha in A431 whole cell lysate. A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab92506 & A431 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92506 in A431 whole cell lysate
    For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/2000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)
    Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)
    Lane 1 : Anti-STAT1 alpha antibody [EPYR2154] (ab92506) at 1/1000 dilution (Purified)
    Lanes 2-3 : Anti-STAT1 alpha antibody [EPYR2154] (ab92506) at 1/1000 dilution

    Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 87 kDa
    Observed band size: 91 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)
    Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)
    All lanes : Anti-STAT1 alpha antibody [EPYR2154] (ab92506) at 1/2000 dilution (unpurified)

    Lane 1 : Untreated HeLa (human cervix adenocarcinoma) membrane
    Lane 2 : HeLa (human cervix adenocarcinoma) membrane treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1500 dilution

    Developed using the ECL technique.

    Predicted band size: 87 kDa
    Observed band size: 91 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Phospho STAT1 protein is revealed by ab109461, anti-STAT1 (phospho S727) antibody. ab92506 is used as pan control which detects total STAT1.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)
    Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)
    All lanes : Anti-STAT1 alpha antibody [EPYR2154] (ab92506) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : NIH-3T3 cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 87 kDa
    Observed band size: 91 kDa why is the actual band size different from the predicted?

  • Anti-STAT1 alpha antibody [EPYR2154] (ab92506)
    Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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