Antibodies, Proteins, Kits and Reagents for Life Science

355 142 ₸ Product size

100 µl 355 142 ₸

Order now and get it on Tuesday March 02, 2021

Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPYR2154] to STAT1 alpha
Suitable for: WB, IP
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-STAT1 alpha antibody [EPYR2154]
See all STAT1 alpha primary antibodies
Description

Rabbit monoclonal [EPYR2154] to STAT1 alpha
Host species

Rabbit
Specificity

Based on Blast results using the immunogen sequence, this antibody should recognise the alpha but not the beta form (only 30% homology) of Stat1.
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

corresponding to Human STAT1 alpha aa 700 to the C-terminus.
Database link: P42224
Positive control
- HeLa, HEK293, NIH-3T3 and A431 cell lysates, mouse brain tissue lysate
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPYR2154
Isotype

IgG
Research areas

Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: STAT1 alpha knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: A431 whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab92506 (unpurified) observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab92506 was shown to specifically react with STAT1 alpha when STAT1 alpha knockout samples were used. Wild-type and STAT1 alpha knockout samples were subjected to SDS-PAGE. Ab92506 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunoprecipitation - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

ab92506 (purified) at 1/20 dilution (0.5ug) immunoprecipitating STAT1 alpha in A431 whole cell lysate. A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab92506 & A431 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92506 in A431 whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/2000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

Lane 1 : Anti-STAT1 alpha antibody [EPYR2154] (ab92506) at 1/1000 dilution (Purified)
Lanes 2-3 : Anti-STAT1 alpha antibody [EPYR2154] (ab92506) at 1/1000 dilution

Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 87 kDa
Observed band size: 91 kDa
why is the actual band size different from the predicted?
Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

All lanes : Anti-STAT1 alpha antibody [EPYR2154] (ab92506) at 1/2000 dilution (unpurified)

Lane 1 : Untreated HeLa (human cervix adenocarcinoma) membrane
Lane 2 : HeLa (human cervix adenocarcinoma) membrane treated with Alkaline Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1500 dilution

Developed using the ECL technique.

Predicted band size: 87 kDa
Observed band size: 91 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Phospho STAT1 protein is revealed by ab109461, anti-STAT1 (phospho S727) antibody. ab92506 is used as pan control which detects total STAT1.
Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

All lanes : Anti-STAT1 alpha antibody [EPYR2154] (ab92506) at 1/1000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : NIH-3T3 cell lysate
Lane 4 : A431 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 87 kDa
Observed band size: 91 kDa why is the actual band size different from the predicted?
Anti-STAT1 alpha antibody [EPYR2154] (ab92506)

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