Anti-MAFK antibody (ab229766)
Key features and details
- Rabbit polyclonal to MAFK
- Suitable for: IP, ChIP, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAFK antibody
See all MAFK primary antibodies -
Description
Rabbit polyclonal to MAFK -
Host species
Rabbit -
Tested applications
Suitable for: IP, ChIP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human MAFK aa 1 to the C-terminus.
Database link: O60675 -
Positive control
- WB: Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MAFK antibody (ab229766)Anti-MAFK antibody (ab229766) at 1/500 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Predicted band size: 18 kDa15% SDS-PAGE gel.
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Immunoprecipitation - Anti-MAFK antibody (ab229766)MAFK was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with 4 μg of ab229766. Western blot was performed from the immunoprecipitate using ab229766 at 1/500 dilution.
Lane 1: Control IgG instead of ab229766 in Jurkat whole cel lysate (input).
Lane 2: ab229766 IP in Jurkat whole cell lysate. -
ChIP - Anti-MAFK antibody (ab229766)Cross-linked ChIP was performed with HepG2 (human liver hepatocellular carcinoma cell line) chromatin extract treated with LPS (10 ng/ml for 1 h) using 5 μg of either normal rabbit IgG or ab229766.
The precipitated DNA was detected by PCR with primer set targeting to IL-8 or GAPDH.
