Anti-Caspase-14 antibody [EPR12927] (ab174847)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12927] to Caspase-14
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-Caspase-14 antibody [EPR12927]
See all Caspase-14 primary antibodies -
Description
Rabbit monoclonal [EPR12927] to Caspase-14 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanIP HumanWB Human -
Immunogen
Synthetic peptide within Human Caspase-14 aa 100-200 (internal sequence). The exact sequence is proprietary.
Database link: P31944 -
Positive control
- MCF7, ECV-304, HACAT and Human skin lysates; fetal skin tissue; Human skin tissue; pellet from fetal skin lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12927 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Caspase-14 antibody [EPR12927] (ab174847) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : ECV-304 cell lysate
Lane 3 : HACAT cell lysate
Lane 4 : Human skin lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 28 kDa
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Immunofluorescent staining of HaCaT cells labeling Caspase-14 (green) with ab174847 at 1:250 dilution, and labeling tubulin (red) with ab7291 at 1:500 (2 µg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) was used at 1:200 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) was used at 1:200.
-ve control 1– Rabbit primary and anti-mouse secondary antibody. -ve control 2 – Mouse primary antibody and anti-rabbit secondary antibody.
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Immunohistochemical analysis of paraffin-embedded human fetal skin tissue labeling Caspase-14 using ab174847 at a 1:100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Caspase-14 using ab174847 at a 1:100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow cytometric analysis of permeabilized HACAT cells labeling Caspase-14 using ab174847 at a 1:10 dilution (red), or rabbit IgG negative control (green).
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Western blot analysis on immunoprecipitation pellet from human fetal skin lysate (lane 1) or 1X PBS (lane 2, negative control) using ab174847 at 1:10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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